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Literature summary for 1.18.1.2 extracted from

  • Balconi, E.; Pennati, A.; Crobu, D.; Pandini, V.; Cerutti, R.; Zanetti, G.; Aliverti, A.
    The ferredoxin-NADP+ reductase/ferredoxin electron transfer system of Plasmodium falciparum (2009), FEBS J., 276, 3825-3836.
    View publication on PubMed
Search for more literature for 1.18.1.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 oxidized ferredoxin Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor Plasmodium falciparum
0.0055
-
 NADPH using cytochrome c as electron acceptor Plasmodium falciparum
0.01
-
 NADPH using K3Fe(CN)6 as electron acceptor Plasmodium falciparum
0.05
-
 NADPH using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum C6KT68
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
 Plasmodium falciparum NADP+ + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
 ?
NADPH + K3Fe(CN)6
-
 Plasmodium falciparum NADP+ + reduced K3Fe(CN)6
-
 r
NADPH + oxidized cytochrome c
-
 Plasmodium falciparum NADP+ + reduced cytochrome c
-
 r
NADPH + oxidized ferredoxin
-
 Plasmodium falciparum NADP+ + reduced ferredoxin
-
 ?
oxidized ferredoxin + NADPH + H+
-
 Plasmodium falciparum reduced ferredoxin + NADP+
-
 r

Subunits

Subunits Comment Organism
More Under oxidizing conditions, Plasmodium falciparum FNR constitutes an inactive dimeric form stabilized by an intermolecular disulfide bond. Monomer-dimer interconversion can be controlled by oxidizing and reducing agents that are possibly present within the apicoplast, such as H2O2, glutathione, and lipoate. Plasmodium falciparum

Synonyms

Synonyms Comment Organism
ferredoxin-NADP+ reductase
-
 Plasmodium falciparum
FNR
-
 Plasmodium falciparum
PfFNR ferredoxin-NADP+ reductase of Plasmodium falciparum Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.5
-
 NADPH using cytochrome c as electron acceptor Plasmodium falciparum
65
-
 NADPH using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor Plasmodium falciparum
85
-
 NADPH using K3Fe(CN)6 as electron acceptor Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1200
-
 NADPH using cytochrome c as electron acceptor Plasmodium falciparum
1300
-
 NADPH using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor Plasmodium falciparum
8000
-
 Ferredoxin Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor Plasmodium falciparum
8500
-
 NADPH using K3Fe(CN)6 as electron acceptor Plasmodium falciparum