Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
H286A | kcat and kcat/Km are markedly reduced | Plasmodium falciparum |
H286K | considerable decrease in kcat and kcat/Km, almost no reactivity with K3Fe(CN)6 | Plasmodium falciparum |
H286L | kcat and kcat/Km are markedly reduced, anomalous reactivity with 2,6-dichlorophenolindophenol | Plasmodium falciparum |
H286Q | up to 50% higher kcat than wild type, higher kcat/Km when 2,6-dichlorophenolindophenol serves as electron acceptor | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-AMP | when 2,6-dichlorophenolindophenol serves as electron acceptor | Plasmodium falciparum | |
K3Fe(CN)6 | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q | Plasmodium falciparum | |
0.036 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor | Plasmodium falciparum | |
0.057 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q | Plasmodium falciparum | |
0.071 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor | Plasmodium falciparum | |
0.14 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K | Plasmodium falciparum | |
0.37 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L | Plasmodium falciparum | |
0.4 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A | Plasmodium falciparum | |
0.52 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | C6KT68 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Plasmodium falciparum | NADP+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
NADPH + K3Fe(CN)6 | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Plasmodium falciparum | NADP+ + reduced K3Fe(CN)6 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ferredoxin-NADP+ reductase | - |
Plasmodium falciparum |
FNR | - |
Plasmodium falciparum |
PfFNR | ferredoxin-NADP+ reductase of Plasmodium falciparum | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286K | Plasmodium falciparum | |
6 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K | Plasmodium falciparum | |
7.5 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L | Plasmodium falciparum | |
17.5 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A | Plasmodium falciparum | |
44.5 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A | Plasmodium falciparum | |
55 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor | Plasmodium falciparum | |
70 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q | Plasmodium falciparum | |
125 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor | Plasmodium falciparum | |
185 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
2'-AMP | when 2,6-dichlorophenolindophenol serves as electron acceptor | Plasmodium falciparum | |
0.23 | - |
K3Fe(CN)6 | - |
Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286L | Plasmodium falciparum | |
45 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286K | Plasmodium falciparum | |
49 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286A | Plasmodium falciparum | |
85 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286A | Plasmodium falciparum | |
750 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor | Plasmodium falciparum | |
3150 | - |
NADPH | in an assay using 2,6-dichlorophenolindophenol as electron acceptor for mutant H286Q | Plasmodium falciparum | |
3300 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor for mutant H286Q | Plasmodium falciparum | |
3500 | - |
NADPH | in an assay using K3Fe(CN)6 as electron acceptor | Plasmodium falciparum |