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Literature summary for 1.18.1.2 extracted from

  • Catalano-Dupuy, D.L.; Orecchia, M.; Rial, D.V.; Ceccarelli, E.A.
    Reduction of the pea ferredoxin-NADP(H) reductase catalytic efficiency by the structuring of a carboxyl-terminal artificial metal binding site (2006), Biochemistry, 45, 13899-13909.
    View publication on PubMed
Search for more literature for 1.18.1.2

Protein Variants

Protein Variants Comment Organism
additional information addition of an artificial metal binding site of nine amino acids, including four His residues, to the C-terminal Tyr308 residue. The additional structure binds Zn2+ or Co2+ and significantly reduces the catalytic efficiency of the enzyme by decreasing the kcat value. In absence of Zn2+, Km value of NADPH and Kd value for NADP+ are increased 2 to 3 times Pisum sativum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 NADPH wild-type, 30°C, pH 8.0 Pisum sativum
0.0024
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, absence of Zn2+, 30°C, pH 8.0 Pisum sativum
0.0026
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, presence of Zn2+, 30°C, pH 8.0 Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
 Pisum sativum NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
 NADPH wild-type, 30°C, pH 8.0 Pisum sativum
10.1
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, presence of Zn2+, 30°C, pH 8.0 Pisum sativum
73.5
-
 NADPH mutant bearing an artificial metal binding site of nine amino acids at the C-terminus, absence of Zn2+, 30°C, pH 8.0 Pisum sativum
74.1
-
 NADPH wild-type, 30°C, pH 8.0 Pisum sativum