Literature summary for 1.17.7.4 extracted from

Wang, W.; Wang, K.; Span, I.; Jauch, J.; Bacher, A.; Groll, M.; Oldfield, E.
Are free radicals involved in IspH catalysis? An EPR and crystallographic investigation (2012), J. Am. Chem. Soc., 134, 11225-11234.

Search for more literature for 1.17.7.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed as a Strep-tagged fusion protein in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
E126Q	inactive mutant used to trap a paramagnetic reaction intermediate representing the 3-CH2OH-rotated weak pi-complex, intermediate II	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified by using Strep-tactin chromatography	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
dimethylallyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+	results show a bioorganometallic mechanism of IspH catalysis. There are two paramagnetic reaction intermediates proposed in this mechanism. The intermediate trapped with IspH E126Q mutants represents the 3-CH2OH-rotated weak pi-complex, intermediate II. Following a formally two-electron reduction and dehydration of the substrate, intermediate II is converted into an eta3-allyl complex with an oxidized HiPIP-like cluster [4Fe-4S]3+, intermediate III, which is observed with wild type IspH	Escherichia coli	a

Synonyms

Synonyms	Comment	Organism
(E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-	Escherichia coli

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Release 2023.1 (January 2023)