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Literature summary for 1.17.7.4 extracted from

  • Wang, K.; Wang, W.; No, J.H.; Zhang, Y.; Zhang, Y.; Oldfield, E.
    Inhibition of the Fe(4)S(4)-cluster-containing protein IspH (LytB): electron paramagnetic resonance, metallacycles, and mechanisms (2010), J. Am. Chem. Soc., 132, 6719-6727.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.17.7.4

Inhibitors

Inhibitors Comment Organism Structure
but-3-ynyl diphosphate
-
 Aquifex aeolicus
additional information analysis of alkynyl diphosphate inhibitors, inhibition model based on an electrostatic interaction with the active site E126, model for alkyne inhibition involving pi (or pi/sigma) metallacycle complex formation with the unique 4th Fe in the Fe4S4 cluster, computational docking and quantum chemical calculations, overview Aquifex aeolicus
propargyl diphosphate
-
 Aquifex aeolicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ IspH contains a [4Fe-4S] cluster Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
 gene ispH or lytB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ HMBPP reductase catalyzes the 2H+/2e- reduction of (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate to form an approximately 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate, insights into IspH catalysis and inhibition, involving organometallic species, overview Aquifex aeolicus dimethylallyl diphosphate + NAD(P)+ + H2O
-
 r
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+ HMBPP reductase catalyzes the 2H+/2e- reduction of (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate to form an approximately 5:1 mixture of isopentenyl diphosphate and dimethylallyl diphosphate, insights into IspH catalysis and inhibition, involving organometallic species, overview Aquifex aeolicus isopentenyl diphosphate + NAD(P)+ + H2O
-
 r

Synonyms

Synonyms Comment Organism
HMBPP reductase
-
 Aquifex aeolicus
ispH
-
 Aquifex aeolicus
LytB
-
 Aquifex aeolicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00045
-
 pH not specified in the publication, temperature not specified in the publication Aquifex aeolicus but-3-ynyl diphosphate