Literature summary for 1.17.4.4 extracted from

Hildebrandt, E.F.; Preusch.P.C.; Patterson, J.L.; Suttie, J.W.
Solubilization and characterization of vitamin K epoxide reductase from normal and warfarin-resistant rat liver microsomes (1984), Arch. Biochem. Biophys., 228, 480-492.

Search for more literature for 1.17.4.4

Activating Compound

Activating Compound	Comment	Organism	Structure
dithiothreitol	-	Rattus norvegicus

General Stability

General Stability	Organism
Deriphat 160, detergent, solubilizes and inactivates	Rattus norvegicus
high ionic strength inactivates during purification	Rattus norvegicus
KCl stabilizes	Rattus norvegicus
omission of salt improves storage stability	Rattus norvegicus
physiological salt concentration stimulates	Rattus norvegicus
Seriphat 160 stabilizes during purification	Rattus norvegicus
sodium cholate, low-concentrated, stabilizes	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
cholate	high concentration	Rattus norvegicus
coumarin anticoagulants	-	Rattus norvegicus
Deriphat 160	detergent, inactivation	Rattus norvegicus
dithiothreitol	high concentration	Rattus norvegicus
warfarin	-	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	vitamin K 2,3-epoxide	-	Rattus norvegicus
0.16	-	dithiothreitol	-	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Rattus norvegicus	-	-
microsome	integral membrane protein	Rattus norvegicus	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K2SO4	activation at high concentration	Rattus norvegicus
KCl	activation at high concentration	Rattus norvegicus
LiCl	activation at high concentration	Rattus norvegicus
NaCl	activation at high concentration	Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol	Rattus norvegicus	-	2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol	-	?
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol	Rattus norvegicus Holtzman	-	2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol	-	?

Organic Solvent Stability

Organic Solvent	Comment	Organism
Ethanol	up to 1% v/v, stable	Rattus norvegicus
Ethanol	1% v/v, stable	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-
Rattus norvegicus	-	Holtzman strain	-
Rattus norvegicus Holtzman	-	Holtzman strain	-

Purification (Commentary)

Purification (Comment)	Organism
-	Rattus norvegicus
partial	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol	-	Rattus norvegicus	2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol	-	?
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol	i.e. vitamin K 2,3-epoxide	Rattus norvegicus	2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol	i.e. 3-hydroxy-2,3-dihydro-vitamin K	?
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol	-	Rattus norvegicus Holtzman	2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol	-	?
2,3-epoxy-2,3-dihydro-2-methyl-3-phytyl-1,4-naphthoquinone + 1,4-dithiothreitol	i.e. vitamin K 2,3-epoxide	Rattus norvegicus Holtzman	2-hydroxy-2-methyl-3-phytyl-2,3-dihydronaphthoquinone + oxidized dithiothreitol	i.e. 3-hydroxy-2,3-dihydro-vitamin K	?
vitamin K 2,3-epoxide + dithiothreitol	-	Rattus norvegicus	vitamin K + oxidized dithiothreitol	-	?
vitamin K 2,3-epoxide + dithiothreitol	-	Rattus norvegicus Holtzman	vitamin K + oxidized dithiothreitol	-	?
vitamin K 2,3-epoxide + reduced dithiothreitol	-	Rattus norvegicus	vitamin K quinone + oxidized dithiothreitol + H2O	-	r
vitamin K 2,3-epoxide + reduced dithiothreitol	-	Rattus norvegicus Holtzman	vitamin K quinone + oxidized dithiothreitol + H2O	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Rattus norvegicus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
7	35	about half-maximal activity at 7°C and 35°C	Rattus norvegicus
18	35	about 55% of maximal activity at 18°C and 35°C	Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-	-	considerably more stable than at 22°C	Rattus norvegicus
-	-	considerably stable	Rattus norvegicus
15	-	rapid inactivation	Rattus norvegicus
22	-	rapid inactivation	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
8.5	9.8	about half-maximal activity at pH 8.5 and 9.8	Rattus norvegicus

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Release 2023.1 (January 2023)