Inhibitors | Comment | Organism | Structure |
---|---|---|---|
caracemide | - |
Escherichia coli | |
caracemide | - |
Homo sapiens | |
caracemide | - |
Lactobacillus leichmannii | |
caracemide | - |
Mus musculus | |
caracemide | - |
Saccharomyces cerevisiae | |
chlorambucil | - |
Escherichia coli | |
chlorambucil | - |
Homo sapiens | |
chlorambucil | - |
Lactobacillus leichmannii | |
chlorambucil | - |
Mus musculus | |
chlorambucil | - |
Saccharomyces cerevisiae | |
cisplatin | - |
Escherichia coli | |
cisplatin | - |
Homo sapiens | |
cisplatin | - |
Lactobacillus leichmannii | |
cisplatin | - |
Mus musculus | |
cisplatin | - |
Saccharomyces cerevisiae | |
Co2+ | RNR activity chelates with copper leading to inactivation | Escherichia coli | |
Co2+ | RNR activity chelates with copper leading to inactivation | Homo sapiens | |
Co2+ | RNR activity chelates with copper leading to inactivation | Lactobacillus leichmannii | |
Co2+ | RNR activity chelates with copper leading to inactivation | Mus musculus | |
Co2+ | RNR activity chelates with copper leading to inactivation | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | class II enzymes contain cobalamin as cofactor | Mus musculus | |
Co2+ | class II enzymes contain cobalamin as cofactor | Escherichia coli | |
Co2+ | class II enzymes contain cobalamin as cofactor | Lactobacillus leichmannii | |
Fe | class I enzymes contain diferric(III)-tyrosyl radical cofactor | Escherichia coli | |
Fe3+ | class I enzymes contain diferric(III)-tyrosyl radical cofactor | Mus musculus | |
Fe3+ | class I enzymes contain diferric(III)-tyrosyl radical cofactor | Homo sapiens | |
Fe3+ | class I enzymes contain diferric(III)-tyrosyl radical cofactor | Saccharomyces cerevisiae | |
Fe3+ | class I enzymes contain diferric(III)-tyrosyl radical cofactor | Lactobacillus leichmannii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | class Ia and Ib RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor. Class II RNRs catalyze the same reaction but also convert nucleoside triphosphates to the correspondent 2'deoxy products, EC 1.17.4.2, overview | ? | - |
? | |
additional information | Escherichia coli | class Ia and Ib RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor. Class II RNRs catalyze the same reaction but also convert nucleoside triphosphates to the correspondent 2'deoxy products, EC 1.17.4.2, overview | ? | - |
? | |
additional information | Lactobacillus leichmannii | class Ia and Ib RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor. Class II RNRs catalyze the same reaction but also convert nucleoside triphosphates to the correspondent 2'deoxy products, EC 1.17.4.2, overview | ? | - |
? | |
additional information | Homo sapiens | class Ia RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor | ? | - |
? | |
additional information | Saccharomyces cerevisiae | class Ia RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor | ? | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | Mus musculus | class I RNRs | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | Escherichia coli | class I RNRs | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | Lactobacillus leichmannii | class I RNRs | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | Homo sapiens | class Ia RNRs | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | Saccharomyces cerevisiae | class Ia RNRs | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + NrdH-redoxin | Mus musculus | only class Ib RNRs | 2'-deoxynucleoside 5'-diphosphate + NrdH-redoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + NrdH-redoxin | Escherichia coli | only class Ib RNRs | 2'-deoxynucleoside 5'-diphosphate + NrdH-redoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + NrdH-redoxin | Lactobacillus leichmannii | only class Ib RNRs | 2'-deoxynucleoside 5'-diphosphate + NrdH-redoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | Mus musculus | class I and class II RNRs | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | Escherichia coli | class I and class II RNRs | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | Lactobacillus leichmannii | class I and class II RNRs | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | Homo sapiens | class Ia RNRs | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | Saccharomyces cerevisiae | class Ia RNRs | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Lactobacillus leichmannii | - |
- |
- |
Mus musculus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | class Ia and Ib RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor. Class II RNRs catalyze the same reaction but also convert nucleoside triphosphates to the correspondent 2'deoxy products, EC 1.17.4.2, overview | Mus musculus | ? | - |
? | |
additional information | class Ia and Ib RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor. Class II RNRs catalyze the same reaction but also convert nucleoside triphosphates to the correspondent 2'deoxy products, EC 1.17.4.2, overview | Escherichia coli | ? | - |
? | |
additional information | class Ia and Ib RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor. Class II RNRs catalyze the same reaction but also convert nucleoside triphosphates to the correspondent 2'deoxy products, EC 1.17.4.2, overview | Lactobacillus leichmannii | ? | - |
? | |
additional information | class Ia RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor | Homo sapiens | ? | - |
? | |
additional information | class Ia RNRs convert nucleoside diphosphates into 2'-deoxynucleoside diphosphates using glutaredoxin or thioredoxin as cofactor | Saccharomyces cerevisiae | ? | - |
? | |
additional information | C-terminus of one monomeric R1 subunit acts in trans to regenerate the active site of its neighboring monomer. The class I RNR active-site disulfide bridge between Cys225 and Cys462 must be reduced for a complete turnover. The electron required for this reduction is provided by a redox network, which involves a cysteine pair at the C-terminus of the R1 subunit, the thioredoxin or glutaredoxin system, and NADPH. For in vitro experiments, the disulfide bridge can be reduced by small thiol compounds such as DTT | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the class I RNR active-site disulfide bridge between Cys225 and Cys462 must be reduced for a complete turnover. The electron required for this reduction is provided by a redox network, which involves a cysteine pair at the C-terminus of the R1 subunit, the thioredoxin or glutaredoxin system, and NADPH. For in vitro experiments, the disulfide bridge can be reduced by small thiol compounds such as DTT | Mus musculus | ? | - |
? | |
additional information | the class I RNR active-site disulfide bridge between Cys225 and Cys462 must be reduced for a complete turnover. The electron required for this reduction is provided by a redox network, which involves a cysteine pair at the C-terminus of the R1 subunit, the thioredoxin or glutaredoxin system, and NADPH. For in vitro experiments, the disulfide bridge can be reduced by small thiol compounds such as DTT | Escherichia coli | ? | - |
? | |
additional information | the class I RNR active-site disulfide bridge between Cys225 and Cys462 must be reduced for a complete turnover. The electron required for this reduction is provided by a redox network, which involves a cysteine pair at the C-terminus of the R1 subunit, the thioredoxin or glutaredoxin system, and NADPH. For in vitro experiments, the disulfide bridge can be reduced by small thiol compounds such as DTT | Homo sapiens | ? | - |
? | |
additional information | the class I RNR active-site disulfide bridge between Cys225 and Cys462 must be reduced for a complete turnover. The electron required for this reduction is provided by a redox network, which involves a cysteine pair at the C-terminus of the R1 subunit, the thioredoxin or glutaredoxin system, and NADPH. For in vitro experiments, the disulfide bridge can be reduced by small thiol compounds such as DTT | Lactobacillus leichmannii | ? | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | class I RNRs | Mus musculus | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | class I RNRs | Escherichia coli | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | class I RNRs | Lactobacillus leichmannii | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | class Ia RNRs | Homo sapiens | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + glutaredoxin | class Ia RNRs | Saccharomyces cerevisiae | 2'-deoxynucleoside 5'-diphosphate + glutaredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + NrdH-redoxin | only class Ib RNRs | Mus musculus | 2'-deoxynucleoside 5'-diphosphate + NrdH-redoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + NrdH-redoxin | only class Ib RNRs | Escherichia coli | 2'-deoxynucleoside 5'-diphosphate + NrdH-redoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + NrdH-redoxin | only class Ib RNRs | Lactobacillus leichmannii | 2'-deoxynucleoside 5'-diphosphate + NrdH-redoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | class I and class II RNRs | Mus musculus | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | class I and class II RNRs | Escherichia coli | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | class I and class II RNRs | Lactobacillus leichmannii | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | class Ia RNRs | Homo sapiens | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? | |
nucleoside 5'-diphosphate + thioredoxin | class Ia RNRs | Saccharomyces cerevisiae | 2'-deoxynucleoside 5'-diphosphate + thioredoxin disulfide + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | alpha or alpha2, class II RNRs | Mus musculus |
monomer or dimer | alpha or alpha2, class II RNRs | Escherichia coli |
monomer or dimer | alpha or alpha2, class II RNRs | Lactobacillus leichmannii |
More | structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview | Mus musculus |
More | structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview | Escherichia coli |
More | structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview | Homo sapiens |
More | structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview | Saccharomyces cerevisiae |
More | structure comparisons of classI-III RNRs, model for the subunit organization of RNRs, overview | Lactobacillus leichmannii |
tetramer | alpha2beta2, class I RNRs | Mus musculus |
tetramer | alpha2beta2, class I RNRs | Escherichia coli |
tetramer | alpha2beta2, class I RNRs | Homo sapiens |
tetramer | alpha2beta2, class I RNRs | Saccharomyces cerevisiae |
tetramer | alpha2beta2, class I RNRs | Lactobacillus leichmannii |
Synonyms | Comment | Organism |
---|---|---|
class Ia RNR | - |
Mus musculus |
class Ia RNR | - |
Escherichia coli |
class Ia RNR | - |
Homo sapiens |
class Ia RNR | - |
Saccharomyces cerevisiae |
class Ia RNR | - |
Lactobacillus leichmannii |
class Ib RNR | - |
Mus musculus |
class Ib RNR | - |
Escherichia coli |
class Ib RNR | - |
Lactobacillus leichmannii |
class II RNR | cf. EC 1.17.4.2 | Mus musculus |
class II RNR | cf. EC 1.17.4.2 | Escherichia coli |
class II RNR | cf. EC 1.17.4.2 | Lactobacillus leichmannii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5'-deoxyadenosylcobalamin | class II enzymes | Escherichia coli | |
Cobalamin | class II enzymes | Mus musculus | |
Cobalamin | class II enzymes | Escherichia coli | |
Cobalamin | class II enzymes | Lactobacillus leichmannii | |
diferric(III)-tyrosyl radical cofactor | class I enzymes | Mus musculus | |
diferric(III)-tyrosyl radical cofactor | class I enzymes | Escherichia coli | |
diferric(III)-tyrosyl radical cofactor | class I enzymes | Homo sapiens | |
diferric(III)-tyrosyl radical cofactor | class I enzymes | Saccharomyces cerevisiae | |
diferric(III)-tyrosyl radical cofactor | class I enzymes | Lactobacillus leichmannii | |
glutaredoxin | class Ia and Ib RNRs | Mus musculus | |
glutaredoxin | class Ia and Ib RNRs | Escherichia coli | |
glutaredoxin | class Ia and Ib RNRs | Lactobacillus leichmannii | |
glutaredoxin | class Ia RNRs | Homo sapiens | |
glutaredoxin | class Ia RNRs | Saccharomyces cerevisiae | |
additional information | cofactor specificity and binding, role in reaction, overview | Mus musculus | |
additional information | cofactor specificity and binding, role in reaction, overview | Escherichia coli | |
additional information | cofactor specificity and binding, role in reaction, overview | Homo sapiens | |
additional information | cofactor specificity and binding, role in reaction, overview | Saccharomyces cerevisiae | |
additional information | cofactor specificity and binding, role in reaction, overview | Lactobacillus leichmannii | |
NrdH-redoxin | class Ib RNRs | Mus musculus | |
NrdH-redoxin | class Ib RNRs | Escherichia coli | |
NrdH-redoxin | class Ib RNRs | Lactobacillus leichmannii | |
thioredoxin | class Ia and Ib RNRs and class II RNRs | Mus musculus | |
thioredoxin | class Ia and Ib RNRs and class II RNRs | Escherichia coli | |
thioredoxin | class Ia and Ib RNRs and class II RNRs | Lactobacillus leichmannii | |
thioredoxin | class Ia RNRs | Homo sapiens | |
thioredoxin | class Ia RNRs | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
additional information | the reaction of class I RNRs involves tyrosyl or cysteinyl radicals and requires aerobic conditions, while for class II RNRs the reaction involves deoxyadenosyl or cysteinyl radicals and is independent of oxygen | Mus musculus |
additional information | the reaction of class I RNRs involves tyrosyl or cysteinyl radicals and requires aerobic conditions, while for class II RNRs the reaction involves deoxyadenosyl or cysteinyl radicals and is independent of oxygen | Lactobacillus leichmannii |
additional information | the reaction of class I RNRs involves tyrosyl or cysteinyl radicals and requires aerobic conditions, while for class II RNRs the reaction involves deoxyadenosyl or cysteinyl radicals and is independent of oxygen. The thiyl radical in class II RNR is believed to be generated directly at the active site using the cofactor 5'-deoxyadenosylcobalamin | Escherichia coli |
additional information | the reaction of class Ia RNRs involves tyrosyl or cysteinyl radicals and requires aerobic conditions | Homo sapiens |
additional information | the reaction of class Ia RNRs involves tyrosyl or cysteinyl radicals and requires aerobic conditions | Saccharomyces cerevisiae |