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Literature summary for 1.17.4.1 extracted from

  • Jiang, W.; Yun, D.; Saleh, L.; Bollinger, J.M.; Krebs, C.
    Formation and function of the manganese(IV)/iron(III) cofactor in Chlamydia trachomatis ribonucleotide reductase (2008), Biochemistry, 47, 13736-13744.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme activation mechanism and kinetics, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ assembly, maintenance, and role in catalysis of the Fe2 III/III-Y radical cofactor of Ecbeta2 subunit, structure modelling, detailed overview Escherichia coli
Fe2+ unusual cofactor instead of Fe-Fe cofactor in other RNRs. Assembly, maintenance, and role in catalysis of the MnIV/FeIII cofactor of Ctbeta2 subunit, structure modelling, detailed overview Chlamydia trachomatis
Mn2+ unusual cofactor instead of Fe-Fe cofactor in other RNRs. Assembly, maintenance, and role in catalysis of the MnIV/FeIII cofactor of Ctbeta2 subunit, structure modelling, detailed overview Chlamydia trachomatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides for DNA synthesis ?
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?
additional information Chlamydia trachomatis ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides for DNA synthesis ?
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?

Organism

Organism UniProt Comment Textmining
Chlamydia trachomatis O84835
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-
Escherichia coli P69924
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides for DNA synthesis Escherichia coli ?
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?
additional information ribonucleotide reductases catalyze the reduction of ribonucleotides to deoxyribonucleotides for DNA synthesis Chlamydia trachomatis ?
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?
additional information the RNR reaction involves replacement by hydrogen of the hydroxyl group on the 2'-carbon of the nucleoside diphosphate substrate. This chemically difficult replacement occurs by a free-radical mechanism. The enzyme employs a heterobinuclear MnIV/FeIII cluster for radical initiation. In essence, the MnIV ion of the cluster functionally replaces the Y radical of the conventional class I RNR. The Ct beta2 protein also autoactivates by reaction of its reduced MnII/FeII metal cluster with O2. In this reaction, an unprecedented MnIV/FeIV intermediate accumulates almost stoichiometrically and decays by one-electron reduction of the FeIV site. This reduction is mediated by the near-surface residue, Y222, overview Escherichia coli ?
-
?
additional information the RNR reaction involves replacement by hydrogen of the hydroxyl group on the 2'-carbon of the nucleoside diphosphate substrate. This chemically difficult replacement occurs by a free-radical mechanism. The enzyme employs a heterobinuclear MnIV/FeIII cluster for radical initiation. In essence, the MnIV ion of the cluster functionally replaces the Y radical of the conventional class I RNR. The Ct beta2 protein also autoactivates by reaction of its reduced MnII/FeII metal cluster with O2. In this reaction, an unprecedented MnIV/FeIV intermediate accumulates almost stoichiometrically and decays by one-electron reduction of the FeIV site. This reduction is mediated by the near-surface residue, Y222, overview Chlamydia trachomatis ?
-
?

Subunits

Subunits Comment Organism
More interaction of the alpha2 and beta2 subunits during the reaction, comparison to the RNR from Chlamydia trachomatis, overview Escherichia coli
More interaction of the alpha2 and beta2 subunits during the reaction, comparison to the RNR from Escherichia coli, overview Chlamydia trachomatis

Synonyms

Synonyms Comment Organism
class I RNR
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Escherichia coli
class I RNR
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Chlamydia trachomatis

Cofactor

Cofactor Comment Organism Structure
Fe2 III/III-Y radical cofactor assembly, maintenance, and role in catalysis of the Fe2 III/III-Y radical cofactor of Ecbeta2 subunit, structure modelling, detailed overview Escherichia coli
Mn/Fe redox cofactor unusual cofactor instead of Fe-Fe cofactor in other RNRs. Assembly, maintenance, and role in catalysis of the MnIV/FeIII cofactor of Ctbeta2 subunit, structure modelling, detailed overview Chlamydia trachomatis