Activating Compound | Comment | Organism | Structure |
---|---|---|---|
O2 | activates the MnIV/FeIII cofactor, overview | Chlamydia trachomatis |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant R2 subunits in Escherichia coli strain BL21(DE3) | Chlamydia trachomatis |
Protein Variants | Comment | Organism |
---|---|---|
W51F | site-directed mutagenesis, the decay of the Mn(IV)/Fe(IV) intermediate is slightly affected | Chlamydia trachomatis |
Y222F | the substitution by site-directed mutagenesis retards the intrinsic decay of the Mn(IV)/Fe(IV) intermediate by about 10fold and diminishes the ability of ascorbate to accelerate the decay by about 65fold but has no detectable effect on the catalytic activity of the Mn(IV)/Fe(III)-R2 product | Chlamydia trachomatis |
Y338F | site-directed mutagenesis, substitution of Y338, the cognate of the subunit interfacial R2 residue in the R1 S R2 PCET pathway of the conventional class I RNRs, has almost no effect on decay of the Mn(IV)/Fe(IV) intermediate but abolishes catalytic activity | Chlamydia trachomatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the class Ic RNR from Chlamydia trachomatis uses a Mn(IV)/Fe(III) cofactor, with high specificity for Mn(IV) in place of the Y for radical initiation, R2 is activated when its MnII/FeII form reacts with O2 to generate a MnIV/FeIV intermediate, which decays by reduction of the FeIV site to the active Mn(IV)/Fe(III) state, the reduction step in this sequence is mediated by residue Y222, overview | Chlamydia trachomatis | |
Manganese | the class Ic RNR from Chlamydia trachomatis uses a Mn(IV)/Fe(III) cofactor, with high specificity for Mn(IV) in place of the Y for radical initiation, R2 is activated when its MnII/FeII form reacts with O2 to generate a MnIV/FeIV intermediate, which decays by reduction of the FeIV site to the active Mn(IV)/Fe(III) state, the reduction step in this sequence is mediated by residue Y222, overview | Chlamydia trachomatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | Chlamydia trachomatis | - |
2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia trachomatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CDP + thioredoxin | - |
Chlamydia trachomatis | 2'-deoxyCDP + thioredoxin disulfide + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNR | - |
Chlamydia trachomatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Chlamydia trachomatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Chlamydia trachomatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
manganese-iron cofactor | the class Ic RNR from Chlamydia trachomatis uses a MnIV/FeIII cofactor, with high specificity for MnIV in place of the tyrosyl radical for radical initiation, R2 is activated when its MnII/FeII form reacts with O2 to generate a MnIV/FeIV intermediate, which decays by reduction of the FeIV site to the active MnIV/FeIII state, the reduction step in this sequence is mediated by residue Y222, overview | Chlamydia trachomatis | |
thioredoxin | - |
Chlamydia trachomatis |