Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | required for CDP reduction | Mus musculus | |
dATP | stimulation of ADP reduction | Mus musculus | |
dATP | 6fold stimulation of GDP reduction | Mus musculus | |
dGTP | required for ADP reduction | Mus musculus | |
dTTP | required for GDP reduction | Mus musculus | |
additional information | subunit R1 has 2 effector-binding sites per polypeptide chain: one activity site for dATP and ATP, with dATP-inhibiting and ATP-stimulating catalytic activity and a second specificity site for dATP, ATP, dTTP and dGTP directing substrate specificity | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D57N | mutation in R1 subunit, in contrast to wild-type dATP stimulates CDP reduction, GDP reduction is inhibited by dGTP, ADP reduction is inhibited by dTTP similar to wild-type, this suggests that the mutant enzyme binds both ATP and dATP to the activity site but does not distinguish between them when it comes to catalysis | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dATP | strong inhibition of the ATP activated enzyme, complete inhibition of GDP reduction, inhibition of ADP reduction | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2'-deoxyribonucleoside 5'-diphosphate + thioredoxin disulfide + H2O = ribonucleoside 5'-diphosphate + thioredoxin | allosteric regulation of the catalytic activity of subunit R1 | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | - |
Mus musculus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | CDP, ADP and GDP are reduced very poorly in the absence of allosteric effectors | ir |