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Literature summary for 1.17.4.1 extracted from
- Ribonucleoside diphosphate reductase (Escherichia coli) (1978), Methods Enzymol., 51, 227-237.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | stimulation of UDP reduction | Escherichia coli |  |
| ATP | stimulation of CDP reduction | Escherichia coli | |
| dATP | stimulation of CDP and UDP reduction | Escherichia coli | |
| dGTP | stimulation of GDP reduction | Escherichia coli | |
| dGTP | stimulation of ADP reduction | Escherichia coli | |
| dTTP | stimulation of UDP reduction | Escherichia coli | |
| dTTP | stimulation of CDP reduction | Escherichia coli | |
| dTTP | stimulation of GDP reduction | Escherichia coli | |
| dTTP | stimulation of ADP reduction | Escherichia coli | |
| additional information | ribonucleoside effectors are exclusively bound at effector binding sites on subunit B1 controlling substrate specificity and activity | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
overproducing strain | - |
| Escherichia coli overproducing | - |
overproducing strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chimeric R2 genes in which C-terminal sequences in Escherichia coli subunit R2 are replaced by C-terminal sequences from the mouse subunit R2 | Escherichia coli |
| overproducing strain | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.59 | - |
purified subunit B1 | Escherichia coli |
| 2.86 | - |
purified subunit B2 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
| ribonucleoside diphosphate + reduced thioredoxin | - |
Escherichia coli overproducing | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha2,beta2, 160000 Da subunit B1 and 78000 Da subunit B2, each consisting of 2 identical or similar proteins | Escherichia coli |
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