Activating Compound | Comment | Organism | Structure |
---|---|---|---|
E. coli thioredoxin reductase | absolute requirement | Tequatrovirus T4 | |
E. coli thioredoxin reductase | enzyme induced in Escherichia coli by infection with bacteriophage T4 | Tequatrovirus T4 | |
thioredoxin reductase | Escherichia coli enzyme | Tequatrovirus T4 |
General Stability | Organism |
---|---|
dithiothreitol, 1 mM, stabilizes | Tequatrovirus T4 |
protein concentrations above 5 mg/ml stabilizes | Tequatrovirus T4 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of different nucleoside triphosphates on Km | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 2.3 atoms of nonheme iron per molecule | Tequatrovirus T4 | |
Mg2+ | 5-10 mM, 2-3fold stimulation, not required for enzyme activity | Tequatrovirus T4 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE | Tequatrovirus T4 |
85000 | - |
alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
enzyme induced in Escherichia coli by infection with bacteriophage T4 | - |
Purification (Comment) | Organism |
---|---|
enzyme induced in Escherichia coli after infection with bacteriophage T4, streptomycin, ammonium sulfate, dATP-Sepharose | Tequatrovirus T4 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.09 | - |
- |
Tequatrovirus T4 |
Storage Stability | Organism |
---|---|
-70°C, 100 mM potassium phosphate, pH 7.0, 1 mM dithiothreitol, enzyme concentration 4.8 mg/ml, 6 months, no loss of activity | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribonucleoside diphosphate + reduced thioredoxin | dithiothreitol at higher concentrations i.e. 100 mM can partially substitute for reduced T4 thioredoxin, the rate of CDP reduction is 10% of that obtained with the complete system | Tequatrovirus T4 | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2, 2 * 85000 + 2 * 35000, enzyme induced in Escherichia coli after infection with bacteriophage T4, SDS-PAGE | Tequatrovirus T4 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Tequatrovirus T4 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | absolute requirement | Tequatrovirus T4 | |
T4 thioredoxin | absolute requirement | Tequatrovirus T4 | |
T4 thioredoxin | enzyme induced in E. coli by infection with bacteriophage T4 | Tequatrovirus T4 |