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Literature summary for 1.17.3.3 extracted from
- Purification and characterization of the molybdoenzymes nicotinate dehydrogenase and 6-hydroxynicotinate dehydrogenase from Bacillus niacini (1990), Arch. Microbiol., 154, 605-613.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| significant loss of activity during the purification procedure, lyophilization results in a complete loss of activity | Neobacillus niacini |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | at a final concentration 10% (v/v), inactivates to 30% of the initial activity | Neobacillus niacini |  |
| KCN | - |
Neobacillus niacini | |
| additional information | not inhibited by NaCl | Neobacillus niacini | |
| Na2S | partially purified 6-HDH, 90% loss of activity | Neobacillus niacini | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
6-Hydroxynicotinate | pH 7.5, 30°C | Neobacillus niacini | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Neobacillus niacini | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | contains iron, iron-sulfur centres | Neobacillus niacini | |
| sulfur | contains acid-labile sulfur, iron-sulfur centres | Neobacillus niacini | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
| 34000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
| 85000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
| 100000 | 140000 | non-denaturing PAGE, gel filtration | Neobacillus niacini |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Neobacillus niacini | acts together with nicotinate dehydrogenase in nicotinate catabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neobacillus niacini | - |
- |
- |
| Neobacillus niacini | - |
strain DSM 2923 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neobacillus niacini |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 6-hydroxynicotinate + H2O + O2 = 2,6-dihydroxynicotinate + H2O2 | contains [2Fe-2S] iron-sulfur centres, FAD and molybdenum, it also has a catalytically essential, labile selenium that can be removed by reaction with cyanide, in Bacillus niacini, this enzyme is required for growth on nicotinic acid | Neobacillus niacini |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.7 | - |
pH 7.5, 30°C | Neobacillus niacini |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-hydroxynicotinate + H2O + 2,6-dichlorophenol indophenol | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced 2,6-dichlorophenol indophenol | - |
? | |
| 6-hydroxynicotinate + H2O + methylene blue | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 40% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced methylene blue | - |
? | |
| 6-hydroxynicotinate + H2O + nitroblue tetrazolium chloride | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced nitroblue tetrazolium chloride | - |
? | |
| 6-hydroxynicotinate + H2O + O2 | - |
Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 | - |
? | |
| 6-hydroxynicotinate + H2O + O2 | highly specific for 6-hydroxynicotinate | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 | - |
? | |
| 6-hydroxynicotinate + H2O + phenazine ethosulfate | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced phenazine ethosulfate | - |
? | |
| 6-hydroxynicotinate + H2O + thiazolyl blue | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced thiazolyl blue | - |
? | |
| 6-hydroxynicotinate + H2O + thionin | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 2% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced thionin | - |
? | |
| additional information | no electron acceptors: Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD+, NADP+, and both viologens | Neobacillus niacini | ? | - |
? | |
| additional information | acts together with nicotinate dehydrogenase in nicotinate catabolism | Neobacillus niacini | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-HDH | - |
Neobacillus niacini |
| 6-hydroxynicotinate hydroxylase | - |
Neobacillus niacini |
| 6-hydroxynicotinic acid dehydrogenase | - |
Neobacillus niacini |
| 6-hydroxynicotinic acid hydroxylase | - |
Neobacillus niacini |
| dehydrogenase, 6-hydroxynicotinate | - |
Neobacillus niacini |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Neobacillus niacini |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Neobacillus niacini |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains FAD | Neobacillus niacini | |
| iron-sulfur centre | contains iron-sulfur centres, iron and acid-labile sulfur | Neobacillus niacini | |
| molybdenum cofactor | contains molybdenum | Neobacillus niacini | |
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