Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Enterobacter cloacae | |
ascorbic acid | - |
Enterobacter cloacae | |
Co2+ | - |
Enterobacter cloacae | |
Cu2+ | - |
Enterobacter cloacae | |
Fe3+ | - |
Enterobacter cloacae | |
H2O2 | - |
Enterobacter cloacae | |
p-chloromercuribenzoate | - |
Enterobacter cloacae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-molybdenum protein | Enterobacter cloacae | |
Molybdenum | an iron-molybdenum protein | Enterobacter cloacae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
2 * 69000, SDS-PAGE | Enterobacter cloacae |
128000 | - |
gel filtration | Enterobacter cloacae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
KY 3074 | - |
Enterobacter cloacae KY 3074 | - |
KY 3074 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | - |
Enterobacter cloacae |
proteolytic modification | - |
Enterobacter cloacae |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.2 | - |
- |
Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-mercaptopurine + H2O + O2 | 4.4% of activity with xanthin | Enterobacter cloacae | 6-mercapto-7,9-dihydropurin-8-one + H2O2 | - |
? | |
adenine + H2O + O2 | no activity with adenine | Enterobacter cloacae | 6-amino-7,9-dihydropurin-8-one + H2O2 | - |
? | |
guanine + H2O + O2 | 53.3% of activity with xanthin | Enterobacter cloacae | 2-amino-7,9-dihydro-1H-purine-6,8-dione + H2O2 | - |
? | |
hypoxanthine + 2 H2O + 2 O2 | 92.3% of activity with xanthin | Enterobacter cloacae | urate + 2 H2O2 | - |
? | |
hypoxanthine + 2 H2O + 2 O2 | 92.3% of activity with xanthin | Enterobacter cloacae KY 3074 | urate + 2 H2O2 | - |
? | |
purine + H2O + O2 | purine and derivatives | Enterobacter cloacae | 7,9-dihydropurin-8-one + H2O2 | - |
? | |
purine + H2O + O2 | 23.3% of activity with xanthine | Enterobacter cloacae | 7,9-dihydropurin-8-one + H2O2 | - |
? | |
purine + H2O + O2 | purine and derivatives | Enterobacter cloacae KY 3074 | 7,9-dihydropurin-8-one + H2O2 | - |
? | |
xanthine + H2O + O2 | electron acceptor O2 | Enterobacter cloacae | uric acid + H2O2 | - |
? | |
xanthine + H2O + O2 | electron acceptor ferricyanide | Enterobacter cloacae | uric acid + H2O2 | - |
? | |
xanthine + H2O + O2 | electron acceptor 2,6-dichlorophenolindophenol | Enterobacter cloacae | uric acid + H2O2 | - |
? | |
xanthine + H2O + O2 | electron acceptor O2 | Enterobacter cloacae KY 3074 | uric acid + H2O2 | - |
? | |
xanthine + H2O + O2 | electron acceptor ferricyanide | Enterobacter cloacae KY 3074 | uric acid + H2O2 | - |
? | |
xanthine + H2O + O2 | electron acceptor 2,6-dichlorophenolindophenol | Enterobacter cloacae KY 3074 | uric acid + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 69000, SDS-PAGE | Enterobacter cloacae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Enterobacter cloacae |
37 | - |
assay at | Enterobacter cloacae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
5°C: about 10% of activity maximum, 60°C: about 50% of activity maximum | Enterobacter cloacae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 6.2-6.8, 30 min, stable | Enterobacter cloacae |
70 | - |
30 min, complete loss of activity | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
- |
Enterobacter cloacae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5.0: about 10% of activity maximum, pH 9.0: about 25% of activity maximum | Enterobacter cloacae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.2 | 6.8 | 60°C, 30 min | Enterobacter cloacae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Enterobacter cloacae |