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Literature summary for 1.17.3.2 extracted from

  • Maia, L.; Mira, L.
    Xanthine oxidase and aldehyde oxidase: A simple procedure for the simultaneous purification from rat liver (2002), Arch. Biochem. Biophys., 400, 48-53.
    View publication on PubMed

General Stability

General Stability Organism
the xanthine dehydrogenase form can be obtained through incubation of xanthine oxidase with sulfhydryl reducing reagents Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
the xanthine dehydrogenase form can be obtained through incubation of xanthine oxidase with sulphydryl reducing reagents
-

Purification (Commentary)

Purification (Comment) Organism
simultaneous purification of xanthine oxidase and aldehyde oxidase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.64
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xanthine + H2O + O2
-
Rattus norvegicus urate + H2O2
-
?

Subunits

Subunits Comment Organism
More on polyacrylamide gel electrophoresis in presence of SDS and 2-mercaptoethanol the purified xanthine oxidase is dissociated into one major band of approximately 150000 Da and three bands of 100000 Da, 60000 Da and 40000 Da Rattus norvegicus

Synonyms

Synonyms Comment Organism
XO
-
Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.8
-
5°C or 10°C, 6 months, no significant loss of activity Rattus norvegicus