General Stability | Organism |
---|---|
lyophilization completely inactivates, high protein concentration may have a stabilizing effect on enzyme activity, sugar protects enzyme from dissociation | Neobacillus niacini |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | after removal of cyanide under anaerobic conditions, enzyme activity can be reconstituted by anaerobic incubation with Na2S | Neobacillus niacini | |
Sulfide | complete loss of activity without previous KCN-treatment | Neobacillus niacini |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
nicotinate | - |
Neobacillus niacini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Neobacillus niacini | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
34000 | - |
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
85000 | - |
x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
280000 | 340000 | gel filtration, non-denaturing PAGE | Neobacillus niacini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + electron acceptor | Neobacillus niacini | first step of nicotinate catabolism | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neobacillus niacini | - |
DSM 2923 | - |
Purification (Comment) | Organism |
---|---|
- |
Neobacillus niacini |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.54 | - |
- |
Neobacillus niacini |
Storage Stability | Organism |
---|---|
-20°C, increase of activity in crude extract | Neobacillus niacini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD(P)+, and both viologens as electron acceptors, not as substrates: 2- or 6-aminonicotinamide, 2-aminonicotinic acid, chelidamic acid, cinchomeronic acid, citrazinic acid, dinicotinic acid, dipicolinic acid, 3-hydroxypicolinic acid, hypoxanthine, isocinchomeronic acid, isonicotinic acid, lutidinic acid, nicotinamide, xanthine | Neobacillus niacini | ? | - |
? | |
nicotinate + H2O + electron acceptor | equilibrium lies far in direction of 6-hydroxynicotinate formation | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
r | |
nicotinate + H2O + electron acceptor | high substrate specificity, transfers electrons only to artificial electron acceptors of high redox potential, i.e. thiazolyl blue, nitroblue tetrazolium chloride, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol, methylene blue with 40% of the activity with phenazine ethosulfate and 2,6-dichlorophenolindophenol | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
r | |
nicotinate + H2O + electron acceptor | first step of nicotinate catabolism | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 85000 + x * 34000 + x * 20000, additional bands with increasing period of storage, subunits are loosely linked by electrostatic interactions, SDS-PAGE | Neobacillus niacini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neobacillus niacini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Neobacillus niacini |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | enzyme contains Fe/S centers, 8.3 mol iron per mol of enzyme, 1.5 mol acid-labile sulfur per mol of enzyme | Neobacillus niacini | |
Bactopterin | enzyme contains bactopterin, an eubacterial modification of molybdopterin, the organic moiety of the molybdenum cofactor | Neobacillus niacini | |
FAD | enzyme contains FAD, 2 mol flavin per mol of enzyme | Neobacillus niacini | |
molybdenum cofactor | enzyme contains 1.5 mol molybdenum per mol of enzyme and bactopterin | Neobacillus niacini |