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Literature summary for 1.17.1.4 extracted from

  • Cao, H.; Pauff, J.; Hille, R.
    Substrate orientation and the origin of catalytic power in xanthine oxidoreductase (2011), Indian J. Chem., 50, 355-362.
No PubMed abstract available
Search for more literature for 1.17.1.4

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli Rhodobacter capsulatus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure determination of the free enzyme and the enzyme in complex with inhibitor alloxanthine Rhodobacter capsulatus

Protein Variants

Protein Variants Comment Organism
R330M the activity with substrate 2-hydroxy-6-methylpurine is only slightly affected Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
alloxanthine a mechanism-based inhibitor, binding structure, overview. Inhibition mechanism involves binding to molybdenum, overview Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ in a [Fe4-S4] domain Rhodobacter capsulatus
Molybdenum
-
 Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
xanthine + NAD+ + H2O = urate + NADH + H+ reaction mechanism, The reaction is initiated by proton abstraction from the Mo-OH group by Glu730, the active-site base, followed by nucleophilic attack on the carbon to be hydroxylated, and hydride transfer to the Mo-S double bond. Suitable substrate orientation, overview. Arg310 is involved in stablizing the transition state in the course of nucleophilic attack, overview Rhodobacter capsulatus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information xanthine and 2-hydroxy-6-methylpurine are substrates. Substrate binding structures, overview Rhodobacter capsulatus ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer alpha2beta2 Rhodobacter capsulatus

General Information

General Information Comment Organism
additional information active site structure, overview Rhodobacter capsulatus