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Literature summary for 1.17.1.4 extracted from

  • Ivanov, N.V.; Trani, M.; Edmondson, D.E.
    High-level expression and characterization of a highly functional Comamonas acidovorans xanthine dehydrogenase in Pseudomonas aeruginosa (2004), Protein Expr. Purif., 37, 72-82.
    View publication on PubMed
Search for more literature for 1.17.1.4

Application

Application Comment Organism
synthesis for maximal level of functional expression, co-expression of xdhC gene is required which increases level of molybdenum incorporation. Iron and FAD content of expressed enzymes are independent of xdhC expression Delftia acidovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron 3.7-3.9 atoms per alphabeta protomer Delftia acidovorans
Molybdenum 1.1 atoms per alphabeta protomer in wild-type, 0.89 atoms per alphabeta protomer after expression of xdhABC genes, 0.24 atoms per alphabeta protomer after expression of xdhAB genes Delftia acidovorans

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
 expression in Pseudomonas aeruginosa, for maximal level of functional expression, co-expression of xdhC gene is required which increases level of molybdenum incorporation. Iron and FAD content of expressed enzymes are independent of xdhC expression
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.58
-
 pH 7.8, 25°C, expression of xdhAB genes Delftia acidovorans
80
-
 pH 7.8, 25°C, expression of xdhABC genes Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
urate + NADH
-
 Delftia acidovorans xanthine + NAD+ + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD 1.0-1.1 molecules per alphabeta protomer Delftia acidovorans