Protein Variants | Comment | Organism |
---|---|---|
E89K | natural mutant strain, lacking iron-sulfur centers, activity to xanthine/NAD+ or xanthine/pterin not affected, but xanthine/phenazine methosulfate activity abolished | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur-center | - |
Drosophila melanogaster | |
molybdenum-center | - |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | Drosophila melanogaster | i.e. pterin | isoxanthopterin + NADH | - |
? | |
xanthine + NAD+ + H2O | Drosophila melanogaster | - |
urate + NADH | - |
? | |
xanthine + O2 + H2O | Drosophila melanogaster | - |
urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
whole body | analysis of a rosy mutant strain | Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.54 | - |
- |
Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-pterin + NAD+ + H2O | i.e. pterin | Drosophila melanogaster | isoxanthopterin + NADH | - |
? | |
2-amino-4-hydroxypterin + NAD+ + H2O | i.e. pterin | Drosophila melanogaster | isoxanthopterin + NADH | - |
? | |
NADH + electron acceptor + H2O | electron acceptor: 2,6-dichlorophenolindophenol, no activity for mutant E89K | Drosophila melanogaster | NAD+ + reduced electron acceptor | - |
? | |
NADH + reduced phenazine methosulfate + cytochrome c | no activity for the mutant E89K | Drosophila melanogaster | NAD+ + ? | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | very low activity for the mutant E89K | Drosophila melanogaster | urate + ? | - |
? | |
xanthine + NAD+ + H2O | - |
Drosophila melanogaster | urate + NADH | - |
? | |
xanthine + O2 + H2O | - |
Drosophila melanogaster | urate + O2- + 2 H+ | - |
? | |
xanthine + phenazine methosulfate + cytochrome c + H2O | very low activity for the mutant E89K | Drosophila melanogaster | urate + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.2 | - |
xanthine | electron acceptor: NAD+ | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Drosophila melanogaster | |
additional information | - |
Drosophila melanogaster | |
NAD+ | - |
Drosophila melanogaster |