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Literature summary for 1.17.1.4 extracted from

  • Hughes, R.K.; Bennett, B.; Bray, R.C.
    Xanthine dehydrogenase from Drosophila melanogaster: Purification and properties of the wild-type enzyme and of a variant lacking iron-sulfur centers (1992), Biochemistry, 31, 3073-3083.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E89K natural mutant strain, lacking iron-sulfur centers, activity to xanthine/NAD+ or xanthine/pterin not affected, but xanthine/phenazine methosulfate activity abolished Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur-center
-
Drosophila melanogaster
molybdenum-center
-
Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-4-hydroxy-pterin + NAD+ + H2O Drosophila melanogaster i.e. pterin isoxanthopterin + NADH
-
?
xanthine + NAD+ + H2O Drosophila melanogaster
-
urate + NADH
-
?
xanthine + O2 + H2O Drosophila melanogaster
-
urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
whole body analysis of a rosy mutant strain Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.54
-
-
Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-pterin + NAD+ + H2O i.e. pterin Drosophila melanogaster isoxanthopterin + NADH
-
?
2-amino-4-hydroxypterin + NAD+ + H2O i.e. pterin Drosophila melanogaster isoxanthopterin + NADH
-
?
NADH + electron acceptor + H2O electron acceptor: 2,6-dichlorophenolindophenol, no activity for mutant E89K Drosophila melanogaster NAD+ + reduced electron acceptor
-
?
NADH + reduced phenazine methosulfate + cytochrome c no activity for the mutant E89K Drosophila melanogaster NAD+ + ?
-
?
xanthine + 2,6-dichlorophenolindophenol + H2O very low activity for the mutant E89K Drosophila melanogaster urate + ?
-
?
xanthine + NAD+ + H2O
-
Drosophila melanogaster urate + NADH
-
?
xanthine + O2 + H2O
-
Drosophila melanogaster urate + O2- + 2 H+
-
?
xanthine + phenazine methosulfate + cytochrome c + H2O very low activity for the mutant E89K Drosophila melanogaster urate + ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.2
-
xanthine electron acceptor: NAD+ Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
FAD
-
Drosophila melanogaster
additional information
-
Drosophila melanogaster
NAD+
-
Drosophila melanogaster