Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.17.1.4 extracted from

  • Gremer, L.; Meyer, O.
    Characterization of xanthine dehydrogenase from the anaerobic bacterium Veillonella atypica and identification of molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor (1996), Eur. J. Biochem., 238, 862-866.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
xanthine electron acceptor: ferricyanide Veillonella atypica

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur-center 1 Fe2-S2-cluster Veillonella atypica
molybdenum-center molybdopterin cytosine dinucleotide cofactor Veillonella atypica
Zn 1.54 mol Zn per mol enzyme Veillonella atypica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica
28500
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica
82400
-
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica
129000
-
native PAGE Veillonella atypica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + O2 + H2O Veillonella atypica very low activity urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Veillonella atypica
-
formerly Micrococcus lactilyticus or Veillonella alcalescens
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Veillonella atypica

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by xanthine Veillonella atypica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
72.56
-
xanthine-ferricyanide assay Veillonella atypica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + ferricyanide + H2O 5% of the activity compared to xanthine Veillonella atypica acetic acid + ferrocyanide
-
?
adenine + ferricyanide + H2O 12% of the activity compared to xanthine Veillonella atypica urate + ferrocyanide
-
?
benzaldehyde + 2 ferricyanide + H2O 4% of the activity compared to xanthine Veillonella atypica benzoate + 2 ferrocyanide + 2 H+
-
?
hypoxanthine + ferricyanide + H2O 98% of the activity compared to xanthine Veillonella atypica xanthine + ferrocyanide
-
?
purine + ferricyanide + H2O 108% of the activity compared to xanthine Veillonella atypica urate + ferrocyanide
-
?
salicylaldehyde + ferricyanide + H2O 2% of the activity compared to xanthine Veillonella atypica salicylate + ferrocyanide
-
?
xanthine + 2,6-dichlorophenolindophenol + H2O 13% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + ?
-
?
xanthine + benzyl viologen + H2O 18% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + reduced benzyl viologen
-
?
xanthine + cytochrome c + H2O low activity Veillonella atypica urate + reduced cytochrome c
-
?
xanthine + FAD + H2O 35% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + FADH2
-
?
xanthine + ferricyanide + H2O
-
Veillonella atypica urate + ferrocyanide
-
?
xanthine + FMN + H2O 44% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + FMNH2
-
?
xanthine + iodonitrotetrazolium + H2O
-
Veillonella atypica urate + reduced iodonitrotetrazolium
-
?
xanthine + methyl viologen + H2O low activity Veillonella atypica urate + reduced methyl viologen
-
?
xanthine + methylene blue + H2O
-
Veillonella atypica urate + reduced methylene blue
-
?
xanthine + nitroblue tetrazolium + H2O
-
Veillonella atypica urate + ?
-
?
xanthine + O2 + H2O very low activity Veillonella atypica urate + O2- + 2 H+
-
?
xanthine + phenazine methosulfate + H2O
-
Veillonella atypica urate + ?
-
?
xanthine + riboflavin + H2O 41% of the activity compared to methylene blue as electron acceptor Veillonella atypica urate + reduced riboflavin
-
?

Subunits

Subunits Comment Organism
trimer 1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE Veillonella atypica

Cofactor

Cofactor Comment Organism Structure
FAD
-
Veillonella atypica
additional information molybdoironflavoprotein: molar ratio of molybdenum to iron to acid-labile-sulfur to FAD is 1 : 2 : 1.9 : 0.8 Veillonella atypica