KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
xanthine | electron acceptor: ferricyanide | Veillonella atypica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur-center | 1 Fe2-S2-cluster | Veillonella atypica | |
molybdenum-center | molybdopterin cytosine dinucleotide cofactor | Veillonella atypica | |
Zn | 1.54 mol Zn per mol enzyme | Veillonella atypica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18400 | - |
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE | Veillonella atypica |
28500 | - |
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE | Veillonella atypica |
82400 | - |
1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE | Veillonella atypica |
129000 | - |
native PAGE | Veillonella atypica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + O2 + H2O | Veillonella atypica | very low activity | urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Veillonella atypica | - |
formerly Micrococcus lactilyticus or Veillonella alcalescens | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Veillonella atypica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by xanthine | Veillonella atypica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72.56 | - |
xanthine-ferricyanide assay | Veillonella atypica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + ferricyanide + H2O | 5% of the activity compared to xanthine | Veillonella atypica | acetic acid + ferrocyanide | - |
? | |
adenine + ferricyanide + H2O | 12% of the activity compared to xanthine | Veillonella atypica | urate + ferrocyanide | - |
? | |
benzaldehyde + 2 ferricyanide + H2O | 4% of the activity compared to xanthine | Veillonella atypica | benzoate + 2 ferrocyanide + 2 H+ | - |
? | |
hypoxanthine + ferricyanide + H2O | 98% of the activity compared to xanthine | Veillonella atypica | xanthine + ferrocyanide | - |
? | |
purine + ferricyanide + H2O | 108% of the activity compared to xanthine | Veillonella atypica | urate + ferrocyanide | - |
? | |
salicylaldehyde + ferricyanide + H2O | 2% of the activity compared to xanthine | Veillonella atypica | salicylate + ferrocyanide | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | 13% of the activity compared to methylene blue as electron acceptor | Veillonella atypica | urate + ? | - |
? | |
xanthine + benzyl viologen + H2O | 18% of the activity compared to methylene blue as electron acceptor | Veillonella atypica | urate + reduced benzyl viologen | - |
? | |
xanthine + cytochrome c + H2O | low activity | Veillonella atypica | urate + reduced cytochrome c | - |
? | |
xanthine + FAD + H2O | 35% of the activity compared to methylene blue as electron acceptor | Veillonella atypica | urate + FADH2 | - |
? | |
xanthine + ferricyanide + H2O | - |
Veillonella atypica | urate + ferrocyanide | - |
? | |
xanthine + FMN + H2O | 44% of the activity compared to methylene blue as electron acceptor | Veillonella atypica | urate + FMNH2 | - |
? | |
xanthine + iodonitrotetrazolium + H2O | - |
Veillonella atypica | urate + reduced iodonitrotetrazolium | - |
? | |
xanthine + methyl viologen + H2O | low activity | Veillonella atypica | urate + reduced methyl viologen | - |
? | |
xanthine + methylene blue + H2O | - |
Veillonella atypica | urate + reduced methylene blue | - |
? | |
xanthine + nitroblue tetrazolium + H2O | - |
Veillonella atypica | urate + ? | - |
? | |
xanthine + O2 + H2O | very low activity | Veillonella atypica | urate + O2- + 2 H+ | - |
? | |
xanthine + phenazine methosulfate + H2O | - |
Veillonella atypica | urate + ? | - |
? | |
xanthine + riboflavin + H2O | 41% of the activity compared to methylene blue as electron acceptor | Veillonella atypica | urate + reduced riboflavin | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 82400 + 1 * 28500 + 1 * 18400, SDS-PAGE | Veillonella atypica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Veillonella atypica | |
additional information | molybdoironflavoprotein: molar ratio of molybdenum to iron to acid-labile-sulfur to FAD is 1 : 2 : 1.9 : 0.8 | Veillonella atypica |