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Literature summary for 1.17.1.3 extracted from

  • Punyasiri, P.A.; Abeysinghe, I.S.; Kumar, V.; Treutter, D.; Duy, D.; Gosch, C.; Martens, S.; Forkmann, G.; Fischer, T.C.
    Flavonoid biosynthesis in the tea plant Camellia sinensis: properties of enzymes of the prominent epicatechin and catechin pathways (2004), Arch. Biochem. Biophys., 431, 22-30.
    View publication on PubMed
Search for more literature for 1.17.1.3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Camellia sinensis enzyme is important in biosynthesis of catchin, epigallocatechin, and anthocyanidins, flavonoid metabolism in tea leaves, overview ?
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 ?

Organism

Organism UniProt Comment Textmining
Camellia sinensis
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-
-

Purification (Commentary)

Purification (Comment) Organism
partially Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf 2 young leaves and bud Camellia sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity of flavonoid metabolism enzymes in tea leaves Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-dihydromyricetin + NADPH combined dihydroflavonol 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis gallocatechin + NADP+ + H2O
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 ?
(2R,3R)-dihydroquercetin + NADPH combined dihydroflavonol 4-reductase/leucoanthocyanidin 4-reductae activity, 2-step reaction Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
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 ?
(2S)-eriodictyol + NADPH flavan formation by combined flavanone 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis luteoliflavan + NADP+ + H2O
-
 ?
(2S)-naringenin + NADPH flavan formation by combined flavanone 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis ?
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 ?
2,3-trans-3,4-cis-leucoanthocyanidin + NADPH
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 Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
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 ?
additional information enzyme is important in biosynthesis of catchin, epigallocatechin, and anthocyanidins, flavonoid metabolism in tea leaves, overview Camellia sinensis ?
-
 ?

Synonyms

Synonyms Comment Organism
LAR
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 Camellia sinensis
leucoanthocyanidin 4-reductase
-
 Camellia sinensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
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 assay at Camellia sinensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at, combined flavanone 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis
7.6
-
 combined dihydroflavonol 4-reductase/leucoanthocyanidin 4-reductae activity Camellia sinensis

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Camellia sinensis