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Literature summary for 1.17.1.1 extracted from

  • Pape, H.; Strominger, J.L.
    Enzymatic synthesis of cytidine diphosphate 3,6-dideoxyhexoses. V. Partial purification of the two protein components required for introduction of the 3-deoxy group (1969), J. Biol. Chem., 244, 3598-3604.
    View publication on PubMed
Search for more literature for 1.17.1.1

Protein Variants

Protein Variants Comment Organism
additional information a mutant which cannot synthesize CDP-abequose is found to be missing E1 Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 89% inhibition at 10 mM Yersinia pseudotuberculosis
iodoacetamide 85% inhibition at 100 mM Yersinia pseudotuberculosis
N-ethylmaleimide 88% inhibition at 10 mM Yersinia pseudotuberculosis
p-chloromercuribenzoate 91% inhibition at 1 mM Yersinia pseudotuberculosis
p-Chlorophenylsulfonate 91% inhibition at 1 mM Yersinia pseudotuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 CDP-4-keto-6-deoxy-D-glucose
-
 Yersinia pseudotuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no divalent cation required Salmonella enterica subsp. enterica serovar Typhimurium
additional information no divalent cation required Yersinia pseudotuberculosis

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2
-
Yersinia pseudotuberculosis
-
 25 VO
-
Yersinia pseudotuberculosis 25VO
-
 25 VO
-

Purification (Commentary)

Purification (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium
enzyme E1: using filtration on a Sephadex G-100 column and column chromatography on DEAE-cellulose, enzyme E3: using two filtrations on Sephadex G-100 columns Yersinia pseudotuberculosis

Reaction

Reaction Comment Organism Reaction ID
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ mechanism Yersinia pseudotuberculosis
a
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ 2 proteins, E1 and E3, are involved but no partial reaction is observed in the presence of either alone Salmonella enterica subsp. enterica serovar Typhimurium
a
CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O = CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H + H+ 2 proteins, E1 and E3, are involved but no partial reaction is observed in the presence of either alone Yersinia pseudotuberculosis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
additional information
-
-
 Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H
-
 Salmonella enterica subsp. enterica serovar Typhimurium CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O
-
 ?
CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H
-
 Yersinia pseudotuberculosis CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O
-
 ?
CDP-4-dehydro-6-deoxy-D-glucose + NAD(P)H
-
 Yersinia pseudotuberculosis 25VO CDP-4-dehydro-3,6-dideoxy-D-glucose + NAD(P)+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
More 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone Salmonella enterica subsp. enterica serovar Typhimurium
More 2 proteins E1 and E3 are involved but no partial reaction has been observed in the presence of either alone Yersinia pseudotuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
-
 Yersinia pseudotuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8.5 pH 5.5: about 60% of activity maximum, pH 8.5: about 40% of activity maximum Yersinia pseudotuberculosis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium
NADPH
-
 Yersinia pseudotuberculosis