Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.16.3.1 extracted from

  • Wang, T.P.; Quintanar, L.; Severance, S.; Solomon, E.I.; Kosman, D.J.
    Targeted suppression of the ferroxidase and iron trafficking activities of the multicopper oxidase Fet3p from Saccharomyces cerevisiae (2003), J. Biol. Inorg. Chem., 8, 611-620.
    View publication on PubMed
Search for more literature for 1.16.3.1

Protein Variants

Protein Variants Comment Organism
D278A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type Saccharomyces cerevisiae
E185A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type, inactive in support of iron uptake Saccharomyces cerevisiae
E185A/Y354A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type Saccharomyces cerevisiae
E185D normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type Saccharomyces cerevisiae
Y354A normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type, in crease in Km-value compared to wild-type Saccharomyces cerevisiae
Y354F normal absorbance at 330 nm and 608 nm due to type 3 and type 1 copper sites, EPR spectra equivalent to wild-type Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
 Fe2+ wild-type, pH 6.0 Saccharomyces cerevisiae
0.005
-
 p-phenylenediamine mutant D278A, pH 6.0 Saccharomyces cerevisiae
0.0058
-
 Fe2+ mutant Y354F, pH 6.0 Saccharomyces cerevisiae
0.008
-
 p-phenylenediamine mutant Y354A, pH 6.0 Saccharomyces cerevisiae
0.0084
-
 p-phenylenediamine wild-type, pH 6.0 Saccharomyces cerevisiae
0.0086
-
 Fe2+ mutant E185D, pH 6.0 Saccharomyces cerevisiae
0.011
-
 Fe2+ mutant D278A, pH 6.0 Saccharomyces cerevisiae
0.0143
-
 p-phenylenediamine mutant Y354F, pH 6.0 Saccharomyces cerevisiae
0.017
-
 Fe2+ mutant Y354A, pH 6.0 Saccharomyces cerevisiae
0.0228
-
 p-phenylenediamine mutant E185D, pH 6.0 Saccharomyces cerevisiae
0.0333
-
 p-phenylenediamine mutant E185A, pH 6.0 Saccharomyces cerevisiae
0.04
-
 Fe2+ mutant E185A, pH 6.0 Saccharomyces cerevisiae
0.0705
-
 p-phenylenediamine mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
3
-
 hydroquinone wild-type, pH 6.0 Saccharomyces cerevisiae
3.51
-
 Fe2+ mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
4.3
-
 hydroquinone mutant D278A, pH 6.0 Saccharomyces cerevisiae
4.6
-
 hydroquinone mutant Y354A, pH 6.0 Saccharomyces cerevisiae
8.2
-
 hydroquinone mutant E185D, pH 6.0 Saccharomyces cerevisiae
12.7
-
 hydroquinone mutant Y354F, pH 6.0 Saccharomyces cerevisiae
14.1
-
 hydroquinone mutant E185A, pH 6.0 Saccharomyces cerevisiae
16.9
-
 hydroquinone mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane outer surface Saccharomyces cerevisiae 5886
-

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroquinone + Fe2+ + O2
-
 Saccharomyces cerevisiae ?
-
 ?
p-phenylenediamine + Fe2+ + O2
-
 Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
multicopper oxidase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 p-phenylenediamine mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
0.39
-
 hydroquinone mutant E185A, pH 6.0 Saccharomyces cerevisiae
0.48
-
 Fe2+ mutant E185A, pH 6.0 Saccharomyces cerevisiae
0.5
-
 Fe2+ mutant D278A, pH 6.0 Saccharomyces cerevisiae
0.52
-
 Fe2+ mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
0.52
-
 hydroquinone mutant E185A/Y354A, pH 6.0 Saccharomyces cerevisiae
0.55
-
 Fe2+ mutant E185D, pH 6.0 Saccharomyces cerevisiae
0.56
-
 Fe2+ mutant Y354A, pH 6.0 Saccharomyces cerevisiae
0.62
-
 hydroquinone mutant D278A, pH 6.0 Saccharomyces cerevisiae
0.62
-
 p-phenylenediamine mutant D278A, pH 6.0 Saccharomyces cerevisiae
0.68
-
 Fe2+ mutant Y354F, pH 6.0 Saccharomyces cerevisiae
0.78
-
 Fe2+ wild-type, pH 6.0 Saccharomyces cerevisiae
0.85
-
 hydroquinone wild-type, pH 6.0 Saccharomyces cerevisiae
0.92
-
 p-phenylenediamine wild-type, pH 6.0 Saccharomyces cerevisiae
0.98
-
 p-phenylenediamine mutant E185A, pH 6.0 Saccharomyces cerevisiae
1.18
-
 p-phenylenediamine mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.57
-
 hydroquinone mutant E185D, pH 6.0 Saccharomyces cerevisiae
1.7
-
 hydroquinone mutant Y354F, pH 6.0 Saccharomyces cerevisiae
1.73
-
 p-phenylenediamine mutant E185D, pH 6.0 Saccharomyces cerevisiae
4.8
-
 p-phenylenediamine mutant Y354A, pH 6.0 Saccharomyces cerevisiae
7.2
-
 hydroquinone mutant Y354A, pH 6.0 Saccharomyces cerevisiae