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Literature summary for 1.16.3.1 extracted from
- Spectral and kinetic properties of the Fet3 protein from Saccharomyces cerevisiae, a multinuclear copper ferroxidase enzyme (1998), J. Biol. Chem., 273, 23274-23282.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| FET3 gene cloned, strain M2 carrying plasmid pDY148 used as expression system, recombinant soluble Fet3p produced in yeast | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
O2 | - |
Saccharomyces cerevisiae |  |
| 0.0013 | - |
O2 | - |
Saccharomyces cerevisiae | |
| 0.002 | - |
Fe2+ | - |
Saccharomyces cerevisiae | |
| 0.0039 | - |
Fe2+ | - |
Homo sapiens | |
| 0.0041 | - |
Fe2+ | - |
Saccharomyces cerevisiae | |
| 0.0048 | - |
Fe2+ | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Homo sapiens | |
| Cu2+ | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61800 | - |
protein characterization | Saccharomyces cerevisiae |
| 72400 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
| 72870 | - |
electrospray mass spectrometry | Saccharomyces cerevisiae |
| 85000 | - |
- |
Homo sapiens |
| 85000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
| 132000 | - |
- |
Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fe2+ + H+ + O2 | Homo sapiens | - |
Fe3+ + H2O | - |
? | |
| Fe2+ + H+ + O2 | Saccharomyces cerevisiae | - |
Fe3+ + H2O | - |
? | |
| Fe2+ + H+ + O2 | Bos taurus | - |
Fe3+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
cow | - |
| Homo sapiens | - |
human | - |
| Saccharomyces cerevisiae | - |
yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble Fet3p | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | venous blood 8 | Homo sapiens | - |
| blood | venous blood 8 | Bos taurus | - |
| plasma | - |
Homo sapiens | - |
| plasma | - |
Saccharomyces cerevisiae | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fe2+ + H+ + O2 | - |
Homo sapiens | Fe3+ + H2O | - |
? | |
| Fe2+ + H+ + O2 | - |
Saccharomyces cerevisiae | Fe3+ + H2O | - |
? | |
| Fe2+ + H+ + O2 | - |
Bos taurus | Fe3+ + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fet3p | - |
Saccharomyces cerevisiae |
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