Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate | detergent, activates | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adriamycin | - |
Rattus norvegicus | |
amiloride | - |
Rattus norvegicus | |
apotransferrin | - |
Rattus norvegicus | |
Atebrin | - |
Rattus norvegicus | |
Chloroquinone | - |
Rattus norvegicus | |
additional information | sensitive to treatment with trypsin | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
NADH | - |
Rattus norvegicus | |
0.033 | - |
Transferrin[Fe(II)]2 | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasmic membrane | - |
Rattus norvegicus | - |
- |
additional information | low activity in the membranes of Golgi apparatus, endoplasmic reticulum and mitochondria, about 10% of the activity in the plasma membrane | Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | potassium phosphate buffer leads to a slightly higher activity than sodium phosphate buffer | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
transferrin[Fe(III)]2 + NADH | Rattus norvegicus | reduction of diferric transferrin at the cell surface may be an important function for diferric transferrin in stimulation of cell growth | transferrin[Fe(II)]2 + NAD+ | - |
? | |
transferrin[Fe(III)]2 + NADH | Rattus norvegicus | role in iron transport | transferrin[Fe(II)]2 + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0057 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferritin[Fe(III)] + NADH | - |
Rattus norvegicus | ferritin[Fe(II)] + NAD+ | - |
? | |
transferrin[Fe(II)]2 + NAD+ + H+ | - |
Rattus norvegicus | transferrin[Fe(III)]2 + NADH | - |
r | |
transferrin[Fe(III)]2 + NADH | - |
Rattus norvegicus | transferrin[Fe(II)]2 + NAD+ | - |
? | |
transferrin[Fe(III)]2 + NADH | reduction of diferric transferrin at the cell surface may be an important function for diferric transferrin in stimulation of cell growth | Rattus norvegicus | transferrin[Fe(II)]2 + NAD+ | - |
? | |
transferrin[Fe(III)]2 + NADH | role in iron transport | Rattus norvegicus | transferrin[Fe(II)]2 + NAD+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | pH 7.0: about 40% of activity maximum, pH 10.0: about 35% of activity maximum | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | preferred electron donor | Rattus norvegicus | |
NADPH | about 50% of the activity with NADH | Rattus norvegicus |