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Literature summary for 1.16.1.10 extracted from

  • Feinberg, L.F.; Holden, J.F.
    Characterization of dissimilatory Fe(III) versus NO3- reduction in the hyperthermophilic archaeon Pyrobaculum aerophilum (2006), J. Bacteriol., 188, 525-531.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pyrobaculum aerophilum 5737
-
membrane
-
Pyrobaculum aerophilum 16020
-

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum
-
-
-
Pyrobaculum aerophilum DSM 7523
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:ferric citrate-grown cell NADH-dependent ferric reductase activity increases approximately 100fold in whole-cell extracts from iron-grown cells relative to those from nitrate-grown cells Pyrobaculum aerophilum
-
culture condition:nitrate-grown cell NADH-dependent ferric reductase activity increases approximately 100fold in whole-cell extracts from iron-grown cells relative to those from nitrate-grown cells Pyrobaculum aerophilum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Fe(III)-ferrozine + NADPH
-
Pyrobaculum aerophilum 2 Fe(II)-ferrozine + NADP+ + H+
-
?
2 Fe(III)-ferrozine + NADPH
-
Pyrobaculum aerophilum DSM 7523 2 Fe(II)-ferrozine + NADP+ + H+
-
?
2 Fe3+ + 3 ferrozine + NADH
-
Pyrobaculum aerophilum 2 Fe(II)-ferrozine + NAD+ + H+
-
?
2 Fe3+ + 3 ferrozine + NADH
-
Pyrobaculum aerophilum DSM 7523 2 Fe(II)-ferrozine + NAD+ + H+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Pyrobaculum aerophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Pyrobaculum aerophilum

Cofactor

Cofactor Comment Organism Structure
NADH rates with NADPH as the electron donor are not significantly different from those with NADH as the donor, activity in cell extract, enzyme not purified Pyrobaculum aerophilum
NADPH rates with NADPH as the electron donor are not significantly different from those with NADH as the donor, activity in cell extract, enzyme not purified Pyrobaculum aerophilum

General Information

General Information Comment Organism
physiological function dissimilatory reduction of iron Pyrobaculum aerophilum