Literature summary for 1.16.1.1 extracted from
Zeyaullah, M.; Haque, S.; Nabi, G.; Nand, K.; Ali, A.
Molecular cloning and expression of bacterial mercuric reductase gene (2010), Afr. J. Biotechnol., 9, 3714-3718.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
plasmid transfer to mercury sensitive Escherichia coli strain DH5alpha, overexpression of gene merA as His-tagged protein in Escherichia coli BL21(DE3)Plys cells |
plasmid R100 |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
66200 |
- |
x * 66200, recombinant His-tagged MerA, SDS-PAGE |
plasmid R100 |
Organism
Organism |
UniProt |
Comment |
Textmining |
plasmid R100 |
- |
mercury-resistant Escherichia coli strain containing plasmid R100 encoding gene merA in the mer operon |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant His-tagged MerA from Escherichia coli strain BL21(DE3)Plys by nickel affinity chromatography |
plasmid R100 |
Subunits
Subunits |
Comment |
Organism |
? |
x * 66200, recombinant His-tagged MerA, SDS-PAGE |
plasmid R100 |
Synonyms
Synonyms |
Comment |
Organism |
MerA |
- |
plasmid R100 |
mercuric reductase |
- |
plasmid R100 |
Cofactor
Cofactor |
Comment |
Organism |
Structure |
FAD |
- |
plasmid R100 |
|
NADPH |
dependent on |
plasmid R100 |
|
General Information
General Information |
Comment |
Organism |
additional information |
MerA is an inducible NADPH-dependent and flavin containing disulfide oxidoreductase enzyme. MerA-encoding plasmid R100-containing Escherichia coli strains are involved in environmental inorganic mercury detoxification |
plasmid R100 |