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Literature summary for 1.15.1.1 extracted from

  • Li, M.; Zhu, L.; Wang, W.
    Improving the thermostability and stress tolerance of an archaeon hyperthermophilic superoxide dismutase by fusion with a unique N-terminal domain (2016), Springerplus, 5, 241.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.15.1.1

Protein Variants

Protein Variants Comment Organism
additional information construction of a fusion protein between the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 and superoxide dismutase from Sulfolobus solfataricus. The recombinant protein exhibits improved thermophilicity, higher working temperature, improved thermostability, broader pH stability, and enhanced tolerance to inhibitors and organic media without any alterations in its oligomerization state. The N-terminal domain is a good candidate for improving stability of both mesophilic and thermophilic superoxide dismutase from either bacteria or archaea via simple genetic manipulation Geobacillus thermodenitrificans
additional information construction of a fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2. The recombinant protein exhibits improved thermophilicity, higher working temperature, improved thermostability, broader pH stability, and enhanced tolerance to inhibitors and organic media without any alterations in its oligomerization state Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A4ISC5
-
-
Geobacillus thermodenitrificans NG80-2 A4ISC5
-
-
Saccharolobus solfataricus P80857
-
-
Saccharolobus solfataricus DSM 1617 P80857
-
-

Synonyms

Synonyms Comment Organism
GTNG_2884
-
 Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 wild-type Saccharolobus solfataricus
60
-
 fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 5 h, 40% loss of activity for wild-type, 13% loss of activity for fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 Saccharolobus solfataricus
105
-
 half-life 2.1 h for wild-type, 5.7 h for fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 both wild-type and fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2, more than 90% of initial activity Saccharolobus solfataricus
5
-
 wild-type, unstable above Saccharolobus solfataricus