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Literature summary for 1.15.1.1 extracted from

  • Kochhar, S.; Kochhar, V.K.
    Identification and characterization of a super-stable Cu-Zn SOD from leaves of turmeric (Curcuma longa L.) (2008), Planta, 228, 307-318.
    View publication on PubMed

General Stability

General Stability Organism
activity of the SOD remains intact both in crude and purified forms after autoclaving at 6-20 bars up to 10 min and microwaving at a frequency of 2450 MHz or million cycles per second for 1-3 min Curcuma longa
polyvinylchloride stabilizes the enzyme Curcuma longa

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition is reversible by Cu and Zn Curcuma longa
H2O2
-
Curcuma longa
KCN
-
Curcuma longa
Sodium diethyldithiocarbamate complete inhibition above 0.1 mM Curcuma longa

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Curcuma longa 9507
-
cytoplasm
-
Curcuma longa 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu a Cu-Zn SOD, activates Curcuma longa
Zn a Cu-Zn SOD Curcuma longa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
2 * 15000, SDS-PAGE Curcuma longa
30800
-
native PAGE Curcuma longa

Organism

Organism UniProt Comment Textmining
Curcuma longa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Curcuma longa
-

Storage Stability

Storage Stability Organism
4°C, purified enzyme, stable for at least 18 months Curcuma longa

Subunits

Subunits Comment Organism
dimer 2 * 15000, SDS-PAGE Curcuma longa

Synonyms

Synonyms Comment Organism
Cu-Zn SOD
-
Curcuma longa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified enzyme, completely stable after 20 min, after 1 h, 98% remaining activity of the leaf enzyme, 88% of the rhizome enzyme Curcuma longa
60
-
purified enzyme, completely stable after 20 min, after 1 h, 90% remaining activity of the leaf enzyme, 70% of the rhizome enzyme Curcuma longa
80
-
purified enzyme, completely stable after 20 min, after 1 h, 80% remaining activity of the leaf enzyme, 52% of the rhizome enzyme Curcuma longa
100
-
purified enzyme, loss of 5% activity after 10 min boiling for the chloroplast enzyme and 35% for the cytosolic enzyme, after 1 h, 70% remaining activity, of the leaf enzyme, 20% of the rhizome enzyme Curcuma longa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Curcuma longa

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.04
-
-
Curcuma longa Sodium diethyldithiocarbamate