Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified zinc-deficient mutant enzyme, 0.002 ml of solution containing 15.7 mg/ml protein in 50 mM Na/K phosphate, pH 7.7, is mixed with 0.002 ml of reservoir solution containing 2.45 M ammonium sulfate, 200 mM NaCl in 50 mM Tris, pH 7.5, room temperature, less than 1 week, X-ray diffraction structure determination and analysis at 2.0 A resolution, modelling | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C111S | site-directed mutagenesis, the mutant has 1.07 copper and 1.18 zinc per subunit | Homo sapiens |
D124N | site-directed mutagenesis, the mutant has 0.93 copper and 0.03 zinc per subunit | Homo sapiens |
D124N/C111S | site-directed mutagenesis, the mutant has 0.93 copper and 0.03 zinc per subunit | Homo sapiens |
D83S | site-directed mutagenesis, the mutant has 0.93 copper and 0.08 zinc per subunit | Homo sapiens |
D83S/C111S | site-directed mutagenesis, the mutant has 0.93 copper and 0.08 zinc per subunit | Homo sapiens |
H80S/D83S | site-directed mutagenesis, the mutant has 0.93 copper and 0.08 zinc per subunit | Homo sapiens |
H80S/D83S/C6A/C111S | site-directed mutagenesis, the mutant has 1.07 copper and 1.18 zinc per subunit | Homo sapiens |
additional information | construction of a zinc-deficient enzyme, structure analysis, the loss of zinc from SOD is potentially important for both the aggregation and zinc-deficient Cu,Zn-SOD hypotheses, and leads to an altered dimer, phenotypes, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a Cu,ZnSOD with 1.07 Cu2+ per enzyme subunit, binding structure, position of Cu2+ in the Zn2+-deficient enzyme A chain active site, overview, physiologic function in Cu,Zn-SOD, overview | Homo sapiens | |
Zn2+ | a Cu,ZnSOD with 1.18 Zn2+ per enzyme subunit, binding structure, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | Homo sapiens | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00441 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2.- + H+ | - |
Homo sapiens | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | asymmetric structure of the zinc-deficient enzyme, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
copper, zinc superoxide dismutase | - |
Homo sapiens |