Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.15.1.1 extracted from

  • Chockalingam, K.; Luba, J.; Nick, H.S.; Silverman, D.N.; Zhao, H.
    Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition (2006), FEBS J., 273, 4853-4861.
    View publication on PubMed
Search for more literature for 1.15.1.1

Protein Variants

Protein Variants Comment Organism
C140S catalytic efficiency similar to wild-type, product inhibition is less than in wild-type Homo sapiens
C140S/Q143A catalysis does not follow Michaelis-Menten kinetics, substrate inhibition with KI-value of 0.06 mM Homo sapiens
N73S ratio kcat/Km about twofold smaller than in wild-type, product inhibition similar to wild-type Homo sapiens
N73S/C140S/Q143A catalytic efficiency much smaller than wild-type, no appreciable product inhibition Homo sapiens
N73S/Q143A catalytic efficiency much smaller than wild-type, no appreciable product inhibition Homo sapiens
Q143A dramatically reduced product inhibition, reduced catalytic activity and efficiency Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
O2- substrate inhibition for mutant C140S/Q143A Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information ratio kcat/Km per microM and s: wild-type, 800, mutant C140S, 800, mutant N73S, 470, mutant Q143A, 3.1, mutant N73S/Q143A, 13, mutant N73S/C140S/Q143A, 15 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform MnSOD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
 Homo sapiens O2 + H2O2
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
 O2- mutant C140S/Q143A, pH 9.0, 25°C Homo sapiens