Protein Variants | Comment | Organism |
---|---|---|
Y34F | unlike wild-type, F- binding is retained at high pH-values. N3- inhibitis Y34F with a 20fold lower KI-value than for wild-type | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HS- | substrate analogue, formation of a green complex upon binding | Escherichia coli | |
N3- | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependence of KM-value reflects the inhibition of anion binding by ionized Y34 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | spectroscopic analysis of reduced and oxidized state of iron. In oxidized state, formation of a six-coordinate complex occurs. Two substrate analogues F- can bind to the oxidized enzymes active site | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AGD3 | FeSOD | - |