Cloned (Comment) | Organism |
---|---|
- |
Cellvibrio japonicus |
Crystallization (Comment) | Organism |
---|---|
to 1.85 A resolution, tri-modular enzyme containing a catalytic family AA10 LPMO module, a family 5 chitin-binding module, and a C-terminal unclassified module which displays tight and specific binding to chitin | Cellvibrio japonicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | removal of the chitin-binding modules reduces LPMO activity toward alpha-chitin. The full-length enzyme and the individual catalytic LPMO module boost the activity of an endochitinase equally well | Cellvibrio japonicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the active site contains a copper ion coordinated by residues His-37 and His-136 in a T-shaped histidine brace | Cellvibrio japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellvibrio japonicus | B3PJ79 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-chitin + ascorbate + O2 | - |
Cellvibrio japonicus | chito-oligosaccharide aldonic acid + dehydroascorbate + H2O | products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation | ? | |
beta-chitin + acceptor + O2 | - |
Cellvibrio japonicus | chito-oligosaccharide aldonic acid + reduced acceptor + H2O | products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation | ? |
Synonyms | Comment | Organism |
---|---|---|
Cbp33A | - |
Cellvibrio japonicus |
LPMO10A | - |
Cellvibrio japonicus |
General Information | Comment | Organism |
---|---|---|
physiological function | the full-length enzyme and the individual catalytic LPMO module boost the activity of an endochitinase equally well, also yielding similar amounts of oxidized products. When grown on insoluble chitin substrates, the deletion mutant shows a reproducible growth defect on beta-chitin leading to an 2fold slower growth rate compared with the wild type strain. When grown on crab shells, the mutation leads to an extended lag phase of about 100 h | Cellvibrio japonicus |