Literature summary for 1.14.99.53 extracted from

Mekasha, S.; Forsberg, Z.; Dalhus, B.; Bacik, J.P.; Choudhary, S.; Schmidt-Dannert, C.; Vaaje-Kolstad, G.; Eijsink, V.G.
Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase from Jonesia denitrificans (2016), FEBS Lett., 590, 34-42.

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Cloned(Commentary)

Cloned (Comment)	Organism
codon-optimized expression in Escherichia coli	Jonesia denitrificans

Crystallization (Commentary)

Crystallization (Comment)	Organism
1.55 A resolution structure of N-terminal LPMO10A module reveals deletions of interacting loops that protrude from the core beta-sandwich scaffold in larger LPMO10s	Jonesia denitrificans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15500	-	-	Jonesia denitrificans

Organism

Organism	UniProt	Comment	Textmining
Jonesia denitrificans	C7R4I0	protein comprises a small N-terminal LPMO10 module named LPMO10A followed by a family 5/12 CBM and a C-terminal GH18 module	-
Jonesia denitrificans DSM 20603	C7R4I0	protein comprises a small N-terminal LPMO10 module named LPMO10A followed by a family 5/12 CBM and a C-terminal GH18 module	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-chitin + ascorbate + O2	-	Jonesia denitrificans	C1-oxidized chitooligosaccharides + dehydroascorbate + H2O	products are C1-oxidized chitooligomers, mainly tetramers and hexamers	?
alpha-chitin + ascorbate + O2	-	Jonesia denitrificans DSM 20603	C1-oxidized chitooligosaccharides + dehydroascorbate + H2O	products are C1-oxidized chitooligomers, mainly tetramers and hexamers	?
beta-chitin + ascorbate + O2	beta-chitin is preferred over alpha-chitin	Jonesia denitrificans	C1-oxidized chitooligosaccharides + dehydroascorbate + H2O	products are C1-oxidized chitooligomers, mainly tetramers and hexamers	?
beta-chitin + ascorbate + O2	beta-chitin is preferred over alpha-chitin	Jonesia denitrificans DSM 20603	C1-oxidized chitooligosaccharides + dehydroascorbate + H2O	products are C1-oxidized chitooligomers, mainly tetramers and hexamers	?
additional information	the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved	Jonesia denitrificans	?	-	?
additional information	the enzyme is active on alpha- and beta-chitin, and the chitin-binding surface previously described for larger LPMOs is fully conserved	Jonesia denitrificans DSM 20603	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 15500, N-terminal LPMO10A module	Jonesia denitrificans

Synonyms

Synonyms	Comment	Organism
Jden_1381	-	Jonesia denitrificans
LPMO10A	-	Jonesia denitrificans

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Release 2023.1 (January 2023)