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Literature summary for 1.14.99.53 extracted from
- A rapid quantitative activity assay shows that the Vibrio cholerae colonization factor GbpA is an active lytic polysaccharide monooxygenase (2014), FEBS Lett., 588, 3435-3440.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | method for quantification of C1-oxidized chitooligosaccharides (aldonic acids), and hence LPMO activity | Vibrio cholerae serotype O1 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Vibrio cholerae serotype O1 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | saturation of enzyme with cu2+ prior to assay | Vibrio cholerae serotype O1 |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio cholerae serotype O1 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-chitin + ascorbate + O2 | - |
Vibrio cholerae serotype O1 | C1-oxidized chitooligosaccharides + dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GbpA | virulence factor with no obvious role in biomass processing | Vibrio cholerae serotype O1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Vibrio cholerae serotype O1 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Vibrio cholerae serotype O1 |
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Release 2023.1 (January 2023)
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