Literature summary for 1.14.99.53 extracted from

Gregory, R.C.; Hemsworth, G.R.; Turkenburg, J.P.; Hart, S.J.; Walton, P.H.; Davies, G.J.
Activity, stability and 3-D structure of the Cu(II) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens (2016), Dalton Trans., 45, 16904-16912.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus amyloliquefaciens

Crystallization (Commentary)

Crystallization (Comment)	Organism
analysis of the copper active site	Bacillus amyloliquefaciens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	Cu(II) binds with KD value 43 nM at pH 5. The coordination geometry around the copper is distorted from axial symmetry	Bacillus amyloliquefaciens
Zn2+	may substitue for Cu2+. KD value 0.0081 mM at pH 5	Bacillus amyloliquefaciens

Organism

Organism	UniProt	Comment	Textmining
Bacillus amyloliquefaciens	-	-	-
Bacillus amyloliquefaciens DSM 7	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
improved expression and purification protocol using Champion pET-SUMO vector, yielding high purity AA10 with yields in excess of 9 mg of protein per litre of culture	Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-chitin + acceptor + O2	-	Bacillus amyloliquefaciens	oligosaccharide aldonic acids + reduced acceptor + H2O	-	?
alpha-chitin + acceptor + O2	-	Bacillus amyloliquefaciens DSM 7	oligosaccharide aldonic acids + reduced acceptor + H2O	-	?
beta-chitin + acceptor + O2	beta chitin from squid pen, best substrate	Bacillus amyloliquefaciens	oligosaccharide aldonic acids + reduced acceptor + H2O	strong preference towards even-numbered products	?
beta-chitin + acceptor + O2	beta chitin from squid pen, best substrate	Bacillus amyloliquefaciens DSM 7	oligosaccharide aldonic acids + reduced acceptor + H2O	strong preference towards even-numbered products	?

Synonyms

Synonyms	Comment	Organism
AA10	-	Bacillus amyloliquefaciens
RBAM17540	-	Bacillus amyloliquefaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	chitin binding increases the thermal stability of the enzyme by 8.3 degrees, copper binding increases melting temperature by 21.6 degrees. Addition of chitin to the copper-bound enzyme increases thermal stability by additional 3.5 degrees	Bacillus amyloliquefaciens
68.7	-	melting temperature, presence of both copper and chitin	Bacillus amyloliquefaciens

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Release 2023.1 (January 2023)