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Literature summary for 1.14.99.46 extracted from

  • Parales, R.; Ingraham, J.
    The surprising Rut pathway: An unexpected way to derive nitrogen from pyrimidines (2010), J. Bacteriol., 192, 4086-4088.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate ?
-
?
thymine + FMNH2 + O2 Escherichia coli
-
(Z)-2-methylureidoacrylate peracid + FMN
-
?
uracil + FMNH2 + O2 Escherichia coli
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(Z)-3-ureidoacrylate peracid + FMN
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P75898 gene rutA, encoded in the rut gene cluster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the pyrimidine ring is cleaved directly by adding a pair of oxygen atoms and producing ureidoacrylate peracid. This odd metabolite, a strong and highly toxic oxidizing agent, is reduced spontaneously, albeit slowly, to ureidoacrylate Escherichia coli ?
-
?
thymine + FMNH2 + O2
-
Escherichia coli (Z)-2-methylureidoacrylate peracid + FMN
-
?
uracil + FMNH2 + O2
-
Escherichia coli (Z)-3-ureidoacrylate peracid + FMN
-
?

Synonyms

Synonyms Comment Organism
RutA
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FMN
-
Escherichia coli

General Information

General Information Comment Organism
metabolism RutA is the first enzyme of the pyrimidine catabolizing Rut pathway, the involved enzymes are encoded by the rut operon, including rutRABCDEFG and ydfG, overview. RutF functions as a flavin reductase that is required to regenerate the flavin mononucleotide, FMN, cofactor Escherichia coli
additional information the Rut pathway degrades exogenous pyrimidines as the sole nitrogen source at room temperature but not at 37°C, a restriction that is apparently a consequence of an inadequate ability to remove toxic malonic semialdehyde at the higher temperature, RutE/YdfG function. The catabolic pathway is highly expressed even in the absence of exogenous substrate and at 37°C Escherichia coli