General Stability | Organism |
---|---|
1 mg/ml albumin stabilizes crude enzyme extract for 1 month when kept frozen | Ilyonectria destructans |
10 h dialysis of purified enzyme against 0.01 M Tris buffer, pH 7.5, 0.005 M EDTA, leads to 50% loss of activity | Ilyonectria destructans |
EDTA affords some protection, especially for crude extracts | Ilyonectria destructans |
preparation is highly unstable | Ilyonectria destructans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | - |
Ilyonectria destructans | |
methylene blue | - |
Ilyonectria destructans | |
p-hydroxymercuribenzoate | - |
Ilyonectria destructans | |
phenazine methosulfate | - |
Ilyonectria destructans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + NADPH + H+ + O2 | Amorphotheca resinae | - |
Testosterone acetate + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | Ilyonectria destructans | - |
Testosterone acetate + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | Ilyonectria destructans ACC 11011 | - |
Testosterone acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amorphotheca resinae | - |
- |
- |
Ilyonectria destructans | - |
ACC 11011 | - |
Ilyonectria destructans ACC 11011 | - |
ACC 11011 | - |
Purification (Comment) | Organism |
---|---|
- |
Ilyonectria destructans |
Storage Stability | Organism |
---|---|
0-6°, 85% of the enzyme activity is lost in 24 h | Ilyonectria destructans |
0°C, 80% enzyme activity is lost in 7 days, half-life of a highly purified preparation is 12 h | Ilyonectria destructans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2 | degradation of pregnane side chains | Ilyonectria destructans | 16alpha-hydroxyandrost-4-ene-3,17-dione | - |
? | |
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2 | degradation of pregnane side chains | Ilyonectria destructans ACC 11011 | 16alpha-hydroxyandrost-4-ene-3,17-dione | - |
? | |
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2 | degradation of pregnane side chains, about 210% the rate of the reaction with progesterone | Ilyonectria destructans | androst-4-ene-3,17-dione + NADP+ + ? | - |
? | |
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2 | degradation of pregnane side chains, about 210% the rate of the reaction with progesterone | Ilyonectria destructans ACC 11011 | androst-4-ene-3,17-dione + NADP+ + ? | - |
? | |
17alpha-methylpregn-4-ene-3,20-dione + NADPH + O2 | about 4.3% the rate of the reaction with progesterone | Ilyonectria destructans | ? | - |
? | |
deoxycorticosterone + NADPH + O2 | degradation of pregnane side chains, about 64% the rate of the reaction with progesterone | Ilyonectria destructans | testosterone + NADP+ + ? | - |
? | |
deoxycorticosterone + NADPH + O2 | degradation of pregnane side chains, about 64% the rate of the reaction with progesterone | Ilyonectria destructans ACC 11011 | testosterone + NADP+ + ? | - |
? | |
additional information | cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates | Ilyonectria destructans | ? | - |
? | |
additional information | cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates | Ilyonectria destructans ACC 11011 | ? | - |
? | |
pregna-4,16-diene-3,20-dione + NADPH + O2 | degradation of pregnane side chains, about 104% the rate of the reaction with progesterone | Ilyonectria destructans | androst-4-ene-3,17-dione | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Amorphotheca resinae | Testosterone acetate + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Ilyonectria destructans | Testosterone acetate + NADP+ + H2O | - |
? | |
progesterone + NADPH + H+ + O2 | - |
Ilyonectria destructans ACC 11011 | Testosterone acetate + NADP+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
progesterone, deoxycorticosterone, 17alpha-hydroxypregn-4-ene-3,20-dione as substrates | Ilyonectria destructans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.4 | 9 | irreversible inactivated below | Ilyonectria destructans |
7 | 8 | most stable | Ilyonectria destructans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | absolute requirement, FMN, FAD, NADH or 2-amino-6,7-dimethyl-4-hydroxy-5,6,7,8-tetrahydropteridine cannot replace NADPH as hydrogen-donor | Ilyonectria destructans |