Cloned (Comment) | Organism |
---|---|
into the pST-Acceptor vector for sequencing, into the vectors pET-15b and pET-28a for expression in Escherichia coli BL21DE3 cells | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
histidine-tagged recombinant DOHH, determined by SDS-PAGE | Plasmodium falciparum |
46450 | - |
theoretical | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodium falciparum | DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | C9QNK6 | - |
- |
Purification (Comment) | Organism |
---|---|
performed by nickel-chelate affinity chromatography under native conditions | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DOHH activity completes hypusine biosynthesis via hydroxylation and thereby completes eukaryotic translation initiation factor eIF-5A formation | Plasmodium falciparum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyhypusine hydroxylase | - |
Plasmodium falciparum |
DOHH | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
activity assay | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
activity assay | Plasmodium falciparum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium falciparum | theoretical | - |
4.96 |