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Literature summary for 1.14.99.24 extracted from

  • Kang, H.K.; Lee, S.S.
    Purification of the NADH reductase component of the steroid 9alpha-hydroxylase from Mycobacterium fortuitum (1997), Arch. Pharm. Res., 20, 590-596.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme catalyzes the production of 9alpha-hydroxy-4-androstene-3,17-dione, an important intermediate for the semi-synthesis of potent anti-inflammatory drugs such as 9alpha-fluorocorticoids from 4-androstene-3,17-dione Mycolicibacterium fortuitum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
NADH
-
Mycolicibacterium fortuitum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Nocardia sp. 5829
-
cytosol
-
Mycolicibacterium fortuitum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Nocardia sp.
Fe2+ iron-sulfur group Mycolicibacterium fortuitum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
NADH reductase component, SDS-PAGE Mycolicibacterium fortuitum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Nocardia sp.
-
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2 Mycolicibacterium fortuitum
-
9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium fortuitum
-
KCTC 1122, ATCC 6842
-
Nocardia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
NADH reductase component Mycolicibacterium fortuitum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.18
-
NADH reductase component Mycolicibacterium fortuitum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
Nocardia sp. 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
?
4,9(11)-pregnadiene-3,20-dione + NADH + O2
-
Mycolicibacterium fortuitum 9,11alpha-epoxypregn-4-ene-3,20-dione + NAD+ + H2O
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Nocardia sp.
FAD
-
Mycolicibacterium fortuitum
NADH
-
Nocardia sp.
NADH
-
Mycolicibacterium fortuitum