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Literature summary for 1.14.99.22 extracted from
- Ecdysone 20-mono-oxygenase in the desert locust, Schistocerca gregaria (1984), Biochem. J., 223, 837-847.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | increase of activity | Schistocerca gregaria |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 20-hydroxyecdysone | competitive inhibition | Schistocerca gregaria | |
| Ca2+ | - |
Schistocerca gregaria | |
| CO | partially reversible by irradiation with monochromatic light | Schistocerca gregaria | |
| EDTA | - |
Schistocerca gregaria | |
| Metyrapone | - |
Schistocerca gregaria | |
| Mg2+ | - |
Schistocerca gregaria | |
| Non-ionic detergents | Tween 20, Tween 80 and Triton X-100 | Schistocerca gregaria | |
| Tris/HCl-buffer | - |
Schistocerca gregaria | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00071 | - |
ecdysone | - |
Schistocerca gregaria | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | membrane bound | Schistocerca gregaria | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ecdysone + NADPH + O2 | Schistocerca gregaria | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schistocerca gregaria | - |
desert locust | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Schistocerca gregaria |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| malpighian tubule | - |
Schistocerca gregaria | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000183 | - |
- |
Schistocerca gregaria |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-ecdysone + NADPH + O2 | - |
Schistocerca gregaria | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
| ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Schistocerca gregaria | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Schistocerca gregaria |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | potassium phosphate buffer | Schistocerca gregaria |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | essential | Schistocerca gregaria | |
| NADH | increase of activity, synergism together with NADPH, but cannot substitute NADPH as cofactor | Schistocerca gregaria | |
| NADPH | - |
Schistocerca gregaria | |
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