General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes activity | Pseudomonas fluorescens |
cysteine stabilizes activity | Pseudomonas fluorescens |
GSH stabilizes activity | Pseudomonas fluorescens |
precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity | Pseudomonas fluorescens |
thioglycolate stabilizes activity | Pseudomonas fluorescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Pseudomonas fluorescens | |
Cu2+ | - |
Pseudomonas fluorescens | |
Hg2+ | - |
Pseudomonas fluorescens | |
iodoacetate | inhibition reversed by addition of GSH or cysteine | Pseudomonas fluorescens | |
Kynurenate | competitive inhibition at 0.2 mM | Pseudomonas fluorescens | |
p-chloromercuribenzoate | inhibition reversed by addition of GSH or cysteine | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
Kynurenate | - |
Pseudomonas fluorescens | |
0.04 | - |
NADH | - |
Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
kynurenate + NADH + O2 | Pseudomonas fluorescens | - |
7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
? | |
kynurenate + NADPH + O2 | Pseudomonas fluorescens | - |
7,8-dihydro-7,8-dihydroxykynurenate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
ATCC 11299b | - |
Purification (Comment) | Organism |
---|---|
partial | Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-10°C, ammonium sulfate fractions, loss of activity after a few days | Pseudomonas fluorescens |
-10°C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
kynurenate + NADH + O2 | - |
Pseudomonas fluorescens | 7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
? | |
kynurenate + NADPH + O2 | - |
Pseudomonas fluorescens | 7,8-dihydro-7,8-dihydroxykynurenate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, complete inactivation | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas fluorescens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
stable in presence 10 mM cysteine | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas fluorescens | |
NADPH | - |
Pseudomonas fluorescens |