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Literature summary for 1.14.99.15 extracted from

  • Bernhardt, F.H.; Erdin, N.; Staudinger, H.; Ullrich, V.
    Interactions of substrates with a purified 4-methoxybenzoate monooxygenase system (O-demethylating) from Pseudomonas putida (1973), Eur. J. Biochem., 35, 126-134.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Putidamonooxin
-
Pseudomonas putida

General Stability

General Stability Organism
NADH, not NADPH or substrate stabilize the reductase Pseudomonas putida
substrate or substrate analogues stabilize putidamomooxin by preventing loss of Fe2+ Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
3-Methoxybenzoate
-
Pseudomonas putida
4-trifluoromethylbenzoate
-
Pseudomonas putida
benzoate competitive inhibition Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0014
-
4-Methoxybenzoate
-
Pseudomonas putida
0.0019
-
O2 + 4-methoxybenzoate Pseudomonas putida
0.0038
-
O2 + 3,4-dimethoxybenzoate Pseudomonas putida
0.01
-
O2 + 4-ethoxybenzoate Pseudomonas putida
0.051
-
O2 + N-methyl-4-aminobenzoate Pseudomonas putida
0.055
-
O2 + 4-methylbenzoate Pseudomonas putida
0.077
-
N-methyl-4-aminobenzoate
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ both enzyme components are iron-sulfur proteins Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methoxybenzoate + NADH + O2 Pseudomonas putida
-
4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
4-hyroxybenzoate, reductase Pseudomonas putida
0.004
-
4-hydroxybenzoate, monooxygenase Pseudomonas putida
0.008
-
3-methoxybenzoate, 4-methoxybenzoate, cell-free extract Pseudomonas putida
0.009
-
N,N-dimethyl-4-aminobenzoate Pseudomonas putida
0.021
-
4-hydroxy-3-methoxybenzoate Pseudomonas putida
0.023
-
3-hydroxybenzoate, reconstituted enzyme Pseudomonas putida
0.024
-
4-ethoxybenzoate, N-methyl-4-aminobenzoate Pseudomonas putida
0.025
-
3,4-dimethoxybenzoate Pseudomonas putida
0.04
-
4-methoxybenzoate, cell-free extract Pseudomonas putida
0.055
-
4-hyxdroxybenzoate Pseudomonas putida
0.077
-
4-hydroxybenzoate, reconstituted enzyme Pseudomonas putida
0.11
-
3-methoxybenzoate Pseudomonas putida
0.178
-
N-methyl-4-aminobenzoate Pseudomonas putida
0.314
-
4-ethoxybenzoate, piperonylate Pseudomonas putida
0.345
-
3,4-dimethoxybenzoate Pseudomonas putida
0.36
-
4-methoxybenzoate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dimethoxybenzoate + NADH + O2
-
Pseudomonas putida 4-hydroxy-3-methoxybenzoate + NAD+ + H2O + formaldehyde
-
?
3,4-methylenedioxybenzoate + NADH + O2 piperonylate Pseudomonas putida 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde protocatechuate ?
3-hydroxybenzoate + NADH + O2 partial uncoupler Pseudomonas putida 3,4-dihydroxybenzoate + NAD+ + H2O in the uncoupled part of the reaction, 3-hydroxybenzoate is not hydroxylated and H2O2 is a product, too ?
3-methoxybenzoate + NADH + O2 partial uncoupler Pseudomonas putida 3-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
4-ethoxybenzoate + NADH + O2
-
Pseudomonas putida 4-hydroxybenzoate + acetaldehyde + NAD+ + H2O
-
?
4-Hydroxy-3-methoxybenzoate + NADH + O2 vanillate, partial uncoupler Pseudomonas putida ?
-
?
4-methoxybenzoate + NADH + O2
-
Pseudomonas putida 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
alkylbenzoates
-
Pseudomonas putida ?
-
?
additional information
-
Pseudomonas putida additional information uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction ?
N,N'-dimethyl-4-aminobenzoate + NADH + O2
-
Pseudomonas putida 4-aminobenzoate + NAD+ + H2O + formaldehyde
-
?
N-methyl-4-aminobenzoate + NADH + O2
-
Pseudomonas putida 4-aminobenzoate + NAD+ + H2O + formaldehyde
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN
-
Pseudomonas putida
NADH
-
Pseudomonas putida