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Literature summary for 1.14.99.15 extracted from

  • Bernhardt, F.H.; Heymann, E.; Traylor, P.S.
    Chemical and spectral properties of putidamonooxin, the iron-containing and acid-labile-sulfur-containing monooxygenase of a 4-methoxybenzoate O-demethylase from Pseudomonas putida (1978), Eur. J. Biochem., 92, 209-223.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
Putidamonooxin
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00007
-
 4-Methoxybenzoate
-
 Pseudomonas putida
0.0003
-
 3-Nitro-4-methoxybenzoate
-
 Pseudomonas putida
0.0058
-
 4-methylaminobenzoate
-
 Pseudomonas putida
0.009
-
 4-Methylbenzoate
-
 Pseudomonas putida
0.024
-
 4-trifluoromethylbenzoate
-
 Pseudomonas putida
0.029
-
 4-hydroxybenzoate
-
 Pseudomonas putida
0.055
-
 O2 + benzoate Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ both enzyme components are iron-sulfur proteins Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
 3-4 * 41500, putidamonooxin, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methoxybenzoate + NADH + O2 Pseudomonas putida
-
 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-nitro-4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
4-hydroxybenzoate + NADH + O2
-
 Pseudomonas putida 3,4-dihydroxybenzoate + NAD+ + H2O
-
 ?
4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
4-methylbenzoate + NADH + O2 p-toluate, partial uncoupler Pseudomonas putida 4-carboxybenzylalcohol + NAD+ + H2O in the uncoupled part of the reaction, p-toluate is not hydroxylated and H2O2 is a product, too ?
4-trifluoromethylbenzoate + NADH + O2
-
 Pseudomonas putida ?
-
 ?
additional information overview: substrates that are not oxygenized while NADH-oxidation and O2-consumption are catalyzed, such as: benzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-hydroxybenzoate, 4-bromobenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-trifluoromethylbenzoate, 4-tert-butylbenzoate Pseudomonas putida additional information uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction ?
N-methyl-4-aminobenzoate + NADH + O2
-
 Pseudomonas putida 4-aminobenzoate + NAD+ + H2O + formaldehyde
-
 ?

Subunits

Subunits Comment Organism
oligomer 3-4 * 41500, putidamonooxin, SDS-PAGE Pseudomonas putida
oligomer 3-4 * 33000-45000, putidamonooxin, SDS-PAGE Pseudomonas putida