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Literature summary for 1.14.99.12 extracted from

  • Itagaki, E.
    Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Oxygenative lactonization of androstenedione to testololactone (1986), J. Biochem., 99, 825-832.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
5-Pregnene-3beta,20alpha-diol
-
 Ilyonectria destructans
dehydroepiandrosterone
-
 Ilyonectria destructans
pregnenolone
-
 Ilyonectria destructans
progesterone
-
 Ilyonectria destructans
testosterone
-
 Ilyonectria destructans
Testosterone acetate
-
 Ilyonectria destructans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 NADPH
-
 Ilyonectria destructans
0.04
-
 androst-4-ene-3,17-dione
-
 Ilyonectria destructans

Organism

Organism UniProt Comment Textmining
Ilyonectria destructans
-
 the steroid monooxygenase also has androstenedione monooxygenase activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
androst-4-ene-3,17-dione + NADPH + O2
-
 Ilyonectria destructans 3-oxo-13,17-secoandrost-4-ene-17,13alpha-lactone + NADP+ + H2O testololactone ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 testololactone formed Ilyonectria destructans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Ilyonectria destructans

pH Range

pH Minimum pH Maximum Comment Organism
5.7 7.8 about 50% of activity maximum at pH 5.7 and 7.8 Ilyonectria destructans

Cofactor

Cofactor Comment Organism Structure
NADPH absolute requirement Ilyonectria destructans