Cloned (Comment) | Organism |
---|---|
expression as glutathione S-transferase fusion protein in Echerichia coli | Streptomyces clavuligerus |
Protein Variants | Comment | Organism |
---|---|---|
R306A | substrate ampicillin, 46%, penicillin G, 37%, phenethicillin, 30%, and carbenicillin, 27% of wild-type activity | Streptomyces clavuligerus |
R306C | substrate ampicillin, 18%, penicillin G, 13%, phenethicillin, 11%, of wild-type activity | Streptomyces clavuligerus |
R306D | substrate ampicillin, 8%, penicillin G, 4%, phenethicillin, 4%, of wild-type activity | Streptomyces clavuligerus |
R306E | substrate ampicillin, 8%, penicillin G, 7%, phenethicillin, 6%,of wild-type activity | Streptomyces clavuligerus |
R306F | substrate ampicillin, 55%, penicillin G, 41%, phenethicillin, 44%, and carbenicillin, 39% of wild-type activity | Streptomyces clavuligerus |
R306G | substrate ampicillin, 21%, penicillin G, 27%, phenethicillin, 19%, of wild-type activity | Streptomyces clavuligerus |
R306H | substrate ampicillin, 16%, penicillin G, 10%, phenethicillin, 8%, of wild-type activity | Streptomyces clavuligerus |
R306I | substrate ampicillin, 168%, penicillin G, 113%, phenethicillin, 149%, and carbenicillin, 167% of wild-type activity | Streptomyces clavuligerus |
R306K | substrate ampicillin, 50%, penicillin G, 53%, phenethicillin, 45%, and carbenicillin, 32% of wild-type activity | Streptomyces clavuligerus |
R306L | substrate ampicillin, 186%, penicillin G, 105%, phenethicillin, 173%, and carbenicillin, 283% of wild-type activity | Streptomyces clavuligerus |
R306M | substrate ampicillin, 113%, penicillin G, 102%, phenethicillin, 115%, and carbenicillin, 122% of wild-type activity | Streptomyces clavuligerus |
R306N | substrate ampicillin, 16%, penicillin G, 14%, phenethicillin, 12%, and carbenicillin, 16% of wild-type activity | Streptomyces clavuligerus |
R306P | no catalytic acitivity | Streptomyces clavuligerus |
R306Q | substrate ampicillin, 62%, penicillin G, 67%, phenethicillin, 54%, and carbenicillin, 62% of wild-type activity | Streptomyces clavuligerus |
R306S | substrate ampicillin, 39%, penicillin G, 37%, phenethicillin, 27%, and carbenicillin, 30% of wild-type activity | Streptomyces clavuligerus |
R306T | substrate ampicillin, 40%, penicillin G, 52%, phenethicillin, 32%, and carbenicillin, 34% of wild-type activity | Streptomyces clavuligerus |
R306V | substrate ampicillin, 34%, penicillin G, 28%, phenethicillin, 36%, and carbenicillin, 32% of wild-type activity | Streptomyces clavuligerus |
R306W | substrate ampicillin, 90%, penicillin G, 61%, phenethicillin, 82%, and carbenicillin, 83% of wild-type activity | Streptomyces clavuligerus |
R306Y | substrate ampicillin, 70%, penicillin G, 59%, phenethicillin, 61%, and carbenicillin, 60% of wild-type activity | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.23 | - |
ampicillin | mutant R306M, 30°C | Streptomyces clavuligerus | |
8.33 | - |
ampicillin | wild-type, 30°C | Streptomyces clavuligerus | |
9.11 | - |
ampicillin | mutant R306I, 30° | Streptomyces clavuligerus | |
10.9 | - |
ampicillin | mutant R306L, 30°C | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | P18548 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.41 | - |
substrate ampicillin, wild-type, 30°C | Streptomyces clavuligerus |
7.23 | - |
substrate ampicillin, mutant R306M, 30°C | Streptomyces clavuligerus |
9.11 | - |
substrate ampicillin, mutant R306I, 30° | Streptomyces clavuligerus |
10.9 | - |
substrate ampicillin, mutant R306L, 30°C | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
carbenicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? | |
phenethicillin + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | ? + succinate + CO2 | - |
? |