Protein Variants | Comment | Organism |
---|---|---|
N305L | improved ability to convert penicillin analogs in ring expansion reaction of EC 1.14.20.1 | Acremonium chrysogenum |
R307L | activities comparable to wild-type | Acremonium chrysogenum |
R308L | improved ability to convert penicillin analogs in ring expansion reaction of EC 1.14.20.1 | Acremonium chrysogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.21 | - |
penicillin G | 30°C, mutant R308L | Acremonium chrysogenum | |
4.51 | - |
penicillin G | 30°C, mutant N305L | Acremonium chrysogenum | |
7.05 | - |
penicillin G | 30°C, mutant R307L | Acremonium chrysogenum | |
7.1 | - |
penicillin G | 30°C, wild-type | Acremonium chrysogenum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acremonium chrysogenum | - |
catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amoxicillin + 2-oxoglutarate + O2 | mutant R308L, 100% of activity, compared to wild-type | Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
ampicillin + 2-oxoglutarate + O2 | mutant N305L, 222%, mutant R307L, 100%, mutant R308L, 311% of activity, compared to wild-type | Acremonium chrysogenum | cephalexin + succinate + CO2 + H2O | - |
? | |
carbenicillin + 2-oxoglutarate + O2 | mutant N305L, 178%, mutant R307L, 93%, mutant R308L, 194% of activity, compared to wild-type | Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? | |
penicillin G + 2-oxoglutarate + O2 | mutant N305L, 163%, mutant R307L, 100%, mutant R308L, 516% of activity, compared to wild-type | Acremonium chrysogenum | phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O | - |
? | |
penicillin V + 2-oxoglutarate + O2 | mutant N305L, 100%, mutant R307L, 93%, mutant R308L, 170% of activity, compared to wild-type | Acremonium chrysogenum | ? + succinate + CO2 + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.071 | - |
penicillin G | 30°C, mutant R307L | Acremonium chrysogenum | |
0.074 | - |
penicillin G | 30°C, mutant N305L | Acremonium chrysogenum | |
0.077 | - |
penicillin G | 30°C, wild-type | Acremonium chrysogenum | |
0.128 | - |
penicillin G | 30°C, mutant R308L | Acremonium chrysogenum |