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Literature summary for 1.14.19.46 extracted from
- Mutation study of conserved amino acid residues of Spirulina DELTA6-acyl-lipid desaturase showing involvement of histidine 313 in the regioselectivity of the enzyme (2004), Appl. Microbiol. Biotechnol., 66, 74-84.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Arthrospira platensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D138N | inactive | Arthrospira platensis |
| E140Q | inactive | Arthrospira platensis |
| G136H | inactive | Arthrospira platensis |
| H124R | inactive | Arthrospira platensis |
| H128R | inactive | Arthrospira platensis |
| H129R | inactive | Arthrospira platensis |
| H305R | the mutant shows 17% of wild type activity | Arthrospira platensis |
| H306R | inactive | Arthrospira platensis |
| H313R | inactive | Arthrospira platensis |
| H315N | inactive | Arthrospira platensis |
| H89R | inactive | Arthrospira platensis |
| H93R | the mutant shows 11% of wild type activity | Arthrospira platensis |
| R123N | the mutant shows 91% of wild type activity | Arthrospira platensis |
| W294G | inactive | Arthrospira platensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
linoleic acid | wild type enzyme, at pH 7.5 and 25°C | Arthrospira platensis |  |
| 0.5 | - |
linoleic acid | mutant enzyme R123N, at pH 7.5 and 25°C | Arthrospira platensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
x * 47000, SDS-PAGE | Arthrospira platensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | Arthrospira platensis | - |
gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrospira platensis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| linoleic acid + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ | - |
Arthrospira platensis | gamma-linolenic acid + oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, SDS-PAGE | Arthrospira platensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA6-acyl-lipid desaturase | - |
Arthrospira platensis |
| desD | - |
Arthrospira platensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Ferredoxin | - |
Arthrospira platensis |
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Release 2023.1 (January 2023)
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