Cloned (Comment) | Organism |
---|---|
gene fad7, DNA and amino acid sequence determination and analysis, sequence comparisons,quantitative real-time PCR enzyme expression analysis, phylogenetic analysis | Camellia sinensis |
gene fad8, DNA and amino acid sequence determination and analysis, sequence comparisons,quantitative real-time PCR enzyme expression analysis, phylogenetic analysis | Camellia sinensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Camellia sinensis | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Camellia sinensis | - |
alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camellia sinensis | L0GGK2 | cv. Longjing43 | - |
Camellia sinensis | S5GIT3 | cv. Longjing43 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Camellia sinensis | - |
additional information | tissue-specific analysis of fatty acid patterns, overview. Enzyme FAD7 is detected in all vegetative tissues, and more abundant in the shoots and mature leaves, which are rich in chlorophyll, expression patterns | Camellia sinensis | - |
additional information | tissue-specific analysis of fatty acid patterns, overview. Enzyme FAD8 is detected in all vegetative tissues, and more abundant in the shoots and mature leaves, which are rich in chlorophyll, expression patterns | Camellia sinensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Camellia sinensis | alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50100, about, sequence calculation | Camellia sinensis |
? | x * 51500, about, sequence calculation | Camellia sinensis |
Synonyms | Comment | Organism |
---|---|---|
FAD7 | - |
Camellia sinensis |
FAD8 | - |
Camellia sinensis |
omega-3 fatty acid desaturase | - |
Camellia sinensis |
plastidial omega-3 desaturase | - |
Camellia sinensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Camellia sinensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Camellia sinensis | sequence calculation | - |
8.1 |
Camellia sinensis | sequence calculation | - |
8.9 |
Organism | Comment | Expression |
---|---|---|
Camellia sinensis | at 4°C, the transcript level of CsFAD7 decreases significantly 1 h after the stress and then recovers. After 6 h, the expression level is of CsFAD7 remains at levels below the control (0 h) until 48 h, at -5°C, CsFAD7 decreases significantly and then remains stable until 48 h | down |
Camellia sinensis | at 4°C, the transcript level of CsFAD8 decreases significantly 1 h after the stress and then recovers. After 6 h, the expression level of FAD8 is higher than the control at 24 h, at -5°C, CsFAD8 expression decreases significantly and then remains stable until 48 h | down |
Camellia sinensis | wounding and abscisic acid induce the enzyme | up |
Camellia sinensis | wounding and abscisic acid induce the enzyme rapidly, while the expression drops below control afterwards | up |
General Information | Comment | Organism |
---|---|---|
physiological function | the plastidial omega-3-fatty acid desaturases CsFAD7 and CsFAD8 are both responsive to abiotic stress signals, but they may play very different roles during stress tolerance in tea plants | Camellia sinensis |