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Literature summary for 1.14.19.35 extracted from

  • Zhang, M.; Barg, R.; Yin, M.; Gueta-Dahan, Y.; Leikin-Frenkel, A.; Salts, Y.; Shabtai, S.; Ben-Hayyim, G.
    Modulated fatty acid desaturation via overexpression of two distinct omega-3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants (2005), Plant J., 44, 361-371.
    View publication on PubMed

Application

Application Comment Organism
agriculture potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance in plants Arabidopsis thaliana
biotechnology potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance in plants Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
gene fad8, recombinant expression in Nicotiana tabacum BY-2 cells and in transgenic tobacco plants via Agrobacterium-mediated transformation. Co-expression of Arabidopsis thaliana FAD8 and Brassica napus FAD3, EC 1.14.19.25, under the control of the 35S CaMV promoter leads to increase in 18:3, which is compensated mainly by the decrease in 18:2, but is also accompanied by a decrease in oleic acid (18:1). Additionally, there is a small but significant decrease in stearic acid (18:0) which seems to be compensated by an increase in palmitic acid (16:0). In the BY-2-FAD8 cells, the ratio of 18:3 to 18:2 is about twofold higher than that in the BY-2 cells. Fatty acid composition of leaf polar lipids of wild-type and transgenic plants, overview Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information recombinant overexpression of FAD8 increases tolerance to drought in tobacco plants and to osmotic stress in cultured cells. Ectopic overexpression of FAD8 induces an increased ratio mainly in the plastidic lipids Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P48622 gene FAD8
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Arabidopsis thaliana Col-0 P48622 gene FAD8
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Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-

Synonyms

Synonyms Comment Organism
FAD8
-
Arabidopsis thaliana

General Information

General Information Comment Organism
additional information ectopic overexpression of FAD8 induces an increased ratio mainly in the plastidic lipids. Overexpression of FAD8 leads to increased tolerance to osmotic stress (imposed by two different agents, PEG 8000 and sorbitol) and decreased tolerance to heat stress (35°C), at both cellular and whole-plant levels. FAD8-overexpressing plants can regain growth after withholding water for a duration that severely impaires the ability of wild-type plants to recover from the stress. Fatty acid composition of leaf polar lipids of wild-type and transgenic plants, overview Arabidopsis thaliana